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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CEACAM1/CD66a Recombinant antibody

CEACAM1/CD66a Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241856H5

Cat no : 84409-4-RR

Synonyms

Biliary glycoprotein D, Biliary glycoprotein 1, BGP-1, Bgp1, Bgp

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CEACAM1/CD66a Recombinant antibody (84409-4-RR)

    WB analysis using 84409-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84409-4-RR (CEACAM-1/CD66a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84409-4-RR

    Affinity and Kinetic Characterization of 84409-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84409-4-RR against Mouse CEACAM1/CD66a were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inmouse liver tissue, mouse small intestine tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84409-4-RR targets CEACAM1/CD66a in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name carcinoembryonic antigen-related cell adhesion molecule 1
Calculated molecular weight57 kDa
Observed molecular weight120 kDa
Gene symbol Ceacam1
Gene ID (NCBI) 26365
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).

Protocols

Product Specific Protocols
WB protocol for CEACAM1/CD66a antibody 84409-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols