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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CLCN5 Polyclonal antibody

CLCN5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Canine, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26812-1-AP

Synonyms

chloride channel 5, Chloride channel protein 5, Chloride transporter ClC 5, ClC 5, CLC5, CLCK2, CLCN5, DENTS, hCIC K2, hClC K2, XLRH, XRN

Validation Data Gallery

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  • Western Blot (WB) analysis of MDCK cells using CLCN5 Polyclonal antibody (26812-1-AP)

    WB analysis of MDCK using 26812-1-AP

    MDCK cells were subjected to SDS PAGE followed by western blot with 26812-1-AP (CLCN5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CLCN5 Polyclonal antibody (26812-1-AP)

    WB analysis of mouse brain using 26812-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26812-1-AP (CLCN5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CLCN5 Polyclonal antibody (26812-1-AP)

    IHC staining of human kidney using 26812-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26812-1-AP (CLCN5 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CLCN5 Polyclonal antibody (26812-1-AP)

    IHC staining of human kidney using 26812-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26812-1-AP (CLCN5 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inMDCK cells, mouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26812-1-AP is CLCN5 Fusion Protein expressed in E. coli.

Tested Reactivity human, Canine, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLCN5 fusion protein Ag25182 相同性解析による交差性が予測される生物種
Full Name chloride channel 5
Calculated molecular weight 83 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC130429
Gene symbol CLCN5
Gene ID (NCBI) 1184
RRIDAB_2880643
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CLCN5 antibody 26812-1-APDownload protocol
IHC protocol for CLCN5 antibody 26812-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Gene Ther

Gene therapy of Dent disease type 1 in newborn ClC-5 null mice for sustained transgene expression and gene therapy effects

Authors - Pin Lyu
View Article