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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Connexin 43 Polyclonal antibody, PBS Only

Connexin 43 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 26980-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Connexin 43 Polyclonal antibody (26980-1-AP)

    WB analysis using 26980-1-AP (same clone as 26980-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26980-1-AP (Connexin 43 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26980-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using Connexin 43 Polyclonal antibody (26980-1-AP)

    WB analysis of mouse testis using 26980-1-AP (same clone as 26980-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 26980-1-AP (Connexin 43 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26980-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using Connexin 43 Polyclonal antibody (26980-1-AP)

    IP experiment of mouse heart using 26980-1-AP (same clone as 26980-1-PBS)

    IP result of anti-Connexin 43 (IP:26980-1-AP, 4ug; Detection:26980-1-AP 1:500) with mouse heart tissue lysate 4000 ug. This data was developed using the same antibody clone with 26980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using Connexin 43 Polyclonal antibody (26980-1-AP)

    IHC staining of rat heart using 26980-1-AP (same clone as 26980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 26980-1-AP (Connexin 43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using Connexin 43 Polyclonal antibody (26980-1-AP)

    IHC staining of rat heart using 26980-1-AP (same clone as 26980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 26980-1-AP (Connexin 43 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Connexin 43 Polyclonal antibody (26980-1-AP)

    IHC staining of mouse heart using 26980-1-AP (same clone as 26980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26980-1-AP (Connexin 43 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Connexin 43 Polyclonal antibody (26980-1-AP)

    IHC staining of human heart using 26980-1-AP (same clone as 26980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26980-1-AP (Connexin 43 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 26980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Connexin 43 Polyclonal antibody (26980-1-AP)

    IHC staining of human heart using 26980-1-AP (same clone as 26980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26980-1-AP (Connexin 43 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 26980-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Connexin 43 Polyclonal antibody (26980-1-AP)

    IF Staining of mouse heart using 26980-1-AP (same clone as 26980-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 26980-1-AP (Connexin 43 antibody) at dilution of 1:50 and 66895-1-Ig (alpha Actinin mouse mAb) at dilution of 1:50, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 26980-1-AP, and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66895-1-Ig. This data was developed using the same antibody clone with 26980-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Connexin 43 Polyclonal antibody (26980-1-AP)

    IF Staining of mouse heart using 26980-1-AP (same clone as 26980-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Connexin 43 antibody (26980-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26980-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human heart tissue using Connexin 43 Polyclonal antibody (26980-1-AP)

    IF Staining of human heart using 26980-1-AP (same clone as 26980-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human heart tissue using 26980-1-AP (Connexin 43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26980-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26980-1-PBS targets Connexin 43 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Connexin 43 fusion protein Ag25533 相同性解析による交差性が予測される生物種
Full Name gap junction protein, alpha 1, 43kDa
Calculated molecular weight 43 kDa
Observed molecular weight 43-47 kDa
GenBank accession numberBC026329
Gene symbol Connexin-43
Gene ID (NCBI) 2697
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Connexin-43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Cx43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).