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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human kidney tissue using GPR141 Polyclonal antibody (28096-1-AP)

    IHC staining of human kidney using 28096-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28096-1-AP (GPR141 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using GPR141 Polyclonal antibody (28096-1-AP)

    IHC staining of human kidney using 28096-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28096-1-AP (GPR141 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GPR141 Polyclonal antibody (28096-1-AP)

    IHC staining of mouse brain using 28096-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28096-1-AP (GPR141 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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GPR141 Polyclonal antibody

GPR141 Polyclonal Antibody for IHC, ELISA
Cat No. 28096-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, ELISA

Synonyms

G protein coupled receptor 141, GPR141, PGR13

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28096-1-AP targets GPR141 in IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR141 fusion protein Ag23129 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 141
Calculated molecular weight 305 aa, 35 kDa
GenBank accession numberBC120951
Gene Symbol GPR141
Gene ID (NCBI) 353345
RRIDAB_3086028
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z602
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPR141 (G-protein-coupled receptor 141) is a class A orphan receptor molecule of the rhodopsin family. Increased GPR141 expression enhances the migratory behavior of breast cancer, driving oncogenic pathways both in vitro and in vivo through activation of epithelial to mesenchymal transition (EMT), oncogenic mediators, and regulation of p-mTOR/p53 signaling (PMID: 37204251).

Protocols

Product Specific Protocols
IHC protocol for GPR141 antibody 28096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols