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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

INSIG1 Polyclonal antibody, PBS Only

INSIG1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 55282-1-PBS

Synonyms

Insulin-induced gene 1 protein, Insulin induced gene 1 protein, insulin induced gene 1, INSIG-1, INSIG 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INSIG1 Polyclonal antibody (55282-1-AP)

    WB analysis using 55282-1-AP (same clone as 55282-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55282-1-AP (INSIG1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55282-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using INSIG1 Polyclonal antibody (55282-1-AP)

    IHC staining of mouse liver using 55282-1-AP (same clone as 55282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55282-1-AP (INSIG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55282-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using INSIG1 Polyclonal antibody (55282-1-AP)

    IHC staining of mouse liver using 55282-1-AP (same clone as 55282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55282-1-AP (INSIG1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55282-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using INSIG1 Polyclonal antibody (55282-1-AP)

    IF Staining of HEK-293 using 55282-1-AP (same clone as 55282-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using INSIG1 antibody (55282-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 55282-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55282-1-PBS targets INSIG1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name insulin induced gene 1
Calculated molecular weight 30 kDa
Observed molecular weight 35 kDa
GenBank accession numberNM_005542
Gene symbol INSIG1
Gene ID (NCBI) 3638
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.