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  • Featured Product
  • KD/KO Validated

KTN1 Polyclonal antibody, PBS Only

KTN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19841-1-PBS

Synonyms

KTN 1, KIAA0004, CG-1 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KTN1 Polyclonal antibody (19841-1-AP)

    WB analysis using 19841-1-AP (same clone as 19841-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19841-1-AP (KTN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19841-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using KTN1 Polyclonal antibody (19841-1-AP)

    WB analysis of A549 using 19841-1-AP (same clone as 19841-1-PBS)

    WB result of KTN1 antibody (19841-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KTN1 transfected A549 cells. This data was developed using the same antibody clone with 19841-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using KTN1 Polyclonal antibody (19841-1-AP)

    IP experiment of Jurkat using 19841-1-AP (same clone as 19841-1-PBS)

    IP result of anti-KTN1 (IP:19841-1-AP, 4ug; Detection:19841-1-AP 1:500) with Jurkat cells lysate 1200ug. This data was developed using the same antibody clone with 19841-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using KTN1 Polyclonal antibody (19841-1-AP)

    IHC staining of human ovary tumor using 19841-1-AP (same clone as 19841-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19841-1-AP (KTN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19841-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using KTN1 Polyclonal antibody (19841-1-AP)

    IHC staining of human colon using 19841-1-AP (same clone as 19841-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 19841-1-AP (KTN1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19841-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bowen disease using KTN1 Polyclonal antibody (19841-1-AP)

    IHC staining of human bowen disease using 19841-1-AP (same clone as 19841-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 19841-1-AP (KTN1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19841-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using KTN1 Polyclonal antibody (19841-1-AP)

    IHC staining of human testis using 19841-1-AP (same clone as 19841-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 19841-1-AP (KTN1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19841-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using KTN1 Polyclonal antibody (19841-1-AP)

    IHC staining of human testis using 19841-1-AP (same clone as 19841-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 19841-1-AP (KTN1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19841-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KTN1 Polyclonal antibody (19841-1-AP)

    IF Staining of HepG2 using 19841-1-AP (same clone as 19841-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KTN1 antibody (19841-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19841-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KTN1 Polyclonal antibody (19841-1-AP)

    IF Staining of HepG2 using 19841-1-AP (same clone as 19841-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KTN1 antibody (19841-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19841-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19841-1-PBS targets KTN1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KTN1 fusion protein Ag13854 相同性解析による交差性が予測される生物種
Full Name kinectin 1 (kinesin receptor)
Calculated molecular weight 1357 aa, 156 kDa
Observed molecular weight 156 kDa
GenBank accession numberBC117132
Gene symbol KTN1
Gene ID (NCBI) 3895
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

KTN1/Kinectin is an integral endoplasmic reticulum (ER) membrane protein which acts as dynamic anchor to facilitate ER membrane extension along microtubules. It has been found to bind kinesin, Rho GTPase, and translation elongation factor-1delta and gets involved in organelle motility and protein synthesis.