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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MTPN Polyclonal antibody

MTPN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13508-1-AP

Synonyms

V 1, Protein V-1, Protein V 1, GCDP

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MTPN Polyclonal antibody (13508-1-AP)

    WB analysis using 13508-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13508-1-AP (MTPN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MTPN Polyclonal antibody (13508-1-AP)

    WB analysis of human brain using 13508-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13508-1-AP (MTPN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human normal colon using MTPN Polyclonal antibody (13508-1-AP)

    IHC staining of human normal colon using 13508-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13508-1-AP (MTPN antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using MTPN Polyclonal antibody (13508-1-AP)

    IHC staining of human heart using 13508-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13508-1-AP (MTPN Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human heart tissue using MTPN Polyclonal antibody (13508-1-AP)

    IHC staining of human heart using 13508-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13508-1-AP (MTPN Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MTPN Polyclonal antibody (13508-1-AP)

    IF Staining of HepG2 using 13508-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MTPN antibody (13508-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MTPN Polyclonal antibody (13508-1-AP)

    IF Staining of HeLa using 13508-1-AP

    Immunofluorescent analysis of Hela cells, using MTPN antibody 13508-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive WB detected inHEK-293T cells, human brain tissue, HeLa cells, Jurkat cells
Positive IHC detected inhuman normal colon, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

13508-1-AP targets MTPN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTPN fusion protein Ag4419 相同性解析による交差性が予測される生物種
Full Name myotrophin
Calculated molecular weight 118 aa, 13 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC028093
Gene symbol MTPN
Gene ID (NCBI) 136319
RRIDAB_2148129
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Myotrophin (MTPN) is a ubiquitously expressed 12 kDa cytoplamic protein that was first isolated from the hearts of spontaneously hypertensive rats. Protein sequence analysis revealed that MTPN was composed of 118 amino acids and was occupied by two and a half internal 33 amino acid ankyrin repeats. MTPN stimulates protein synthesis and increases the expression of a number of cardiac marker genes (such as atrial natriuretic factor and b-myosin heavy chain) in cardiomyocytes leading to hypertrophy. In skeletal muscle, MTPN is also a positive growth factor in promoting skeletal muscle growth.

Protocols

Product Specific Protocols
WB protocol for MTPN antibody 13508-1-APDownload protocol
IHC protocol for MTPN antibody 13508-1-APDownload protocol
IF protocol for MTPN antibody 13508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Am J Obstet Gynecol

Trophoblast apoptosis from pregnancies complicated by fetal growth restriction is associated with enhanced p53 expression.

Authors - Roni Levy
View Article
humanIHC

J Clin Biochem Nutr

Upregulation of ENDOU in cytotrophoblasts from placenta complicated with preeclampsia and fetal growth restriction.

Authors - Masataka Nomoto
View Article