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PSD95-Specific,DLG4 Polyclonal antibody

PSD95-Specific,DLG4 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20665-1-AP

Synonyms

Disks large homolog 4, DLG4, PSD 95, PSD95, PSD95-Specific,DLG4, SAP 90, SAP90, Synapse associated protein 90



Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inNeuro-2a cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

20665-1-AP targets PSD95-Specific,DLG4 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse, zebrafish, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name discs, large homolog 4 (Drosophila)
Calculated molecular weight 85 kDa
Observed molecular weight 90-95 kDa
GenBank accession numberNM_001365
Gene symbol DLG4
Gene ID (NCBI) 1742
RRIDAB_2687961
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (disks large homolog 4) gene. PSD-95 is a scaffolding protein of the MAGUK protein family, and engages in several vital protein-protein interactions in the brain with its PDZ domains. It has been suggested that PSD-95 is composed of two supramodules, one of which is the PDZ1-2 tandem domain. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation. Observed MW of PSD95 is 90-95 kDa due to phosphorylation (PMID: 20682303).

Protocols

Product Specific Protocols
WB protocol for PSD95-Specific,DLG4 antibody 20665-1-APDownload protocol
IHC protocol for PSD95-Specific,DLG4 antibody 20665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Nat Neurosci

Foxp2 controls synaptic wiring of corticostriatal circuits and vocal communication by opposing Mef2c.

Authors - Yi-Chuan Chen
mouseWB

Neuron

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Authors - Xiaohui Li
mouseWB,IF

Neuron

Selective removal of astrocytic APOE4 strongly protects against tau-mediated neurodegeneration and decreases synaptic phagocytosis by microglia.

Authors - Chao Wang
mouseWB

Sci Adv

Loss of schizophrenia-related miR-501-3p in mice impairs sociability and memory by enhancing mGluR5-mediated glutamatergic transmission

Authors - Wenquan Liang
mouseWB

Sci Adv

RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission

Authors - Pengcheng Ma
mouseWB

Mol Psychiatry

A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.

Authors - Yue Qin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tatyana (Verified Customer) (03-11-2023)

Works well in IHC and WB in mouse brain. WB: 4% milk in TBST and semi-dry transfer; detects a double band at ~100kDa. IHC: 4% PFA fixed and paraffinized sections; standard antigen retrieval and DAB detection. Used at 1:1000, with incubation overnight at 4C.

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse, CNS tissue
PSD95-Specific,DLG4 Antibody Western Blot,Immunohistochemistry validation (1:1000 dilution) in Mouse, CNS tissue (Cat no:20665-1-AP)
FH

Lisa (Verified Customer) (03-17-2022)

The antibody works well for WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1500
  • Cell Tissue Type: hiPSCs-derived neurons
FH

James (Verified Customer) (09-29-2021)

Antibody was not as specific as I had hoped. I used the antibody at 1:1000, I detected a band at 95kDa which is PSD-95, however, other bands were also detected. I will be looking to purchase a more specific monoclonal antibody for my next experiments as the specificity is an issue for me.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Neurogenin 2 iPSC Neurons Day 21
PSD95-Specific,DLG4 Antibody Western Blot validation (1:1000 dilution) in Neurogenin 2 iPSC Neurons Day 21 (Cat no:20665-1-AP)