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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMIM1 Polyclonal antibody

SMIM1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27849-1-AP

Synonyms

hypothetical LOC388588, LOC388588, Small integral membrane protein 1, SMIM1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SMIM1 Polyclonal antibody (27849-1-AP)

    WB analysis of HEK-293 using 27849-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 27849-1-AP (SMIM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SMIM1 Polyclonal antibody (27849-1-AP)

    WB analysis using 27849-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27849-1-AP (SMIM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SMIM1 Polyclonal antibody (27849-1-AP)

    WB analysis of mouse kidney using 27849-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27849-1-AP (SMIM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27849-1-AP targets SMIM1 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMIM1 fusion protein Ag27028 相同性解析による交差性が予測される生物種
Full Name hypothetical LOC388588
Observed molecular weight 9 kDa
GenBank accession numberBC146957
Gene symbol SMIM1
Gene ID (NCBI) 388588
RRIDAB_2880993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Small integral membrane protein 1(SMIM1) is a type II transmembrane phosphoprotein and displays the Vel blood group antigen at its carboxyl-terminus. SMIM1 is highly expressed in hematopoietic cells. It is associated with a variety of immune responses and plays an important role in RBC differentiation. SMIM1 can be used as a potential biomarker for the diagnosis of IDD (PMID: 30906890; 26452714).

Protocols

Product Specific Protocols
WB protocol for SMIM1 antibody 27849-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Transfus Med Hemother

CRISPR/Cas9-Editing K562 Cell Line as a Potential Tool in Transfusion Applications: Knockout of Vel Antigen Gene

Authors - Jiaxuan Yang
  • KO Validated
View Article