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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WBP11 Polyclonal antibody

WBP11 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10316-1-AP

Synonyms

SNP70, SIPP1, SH3 domain-binding protein SNP70, FAP90, CFAP90

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WBP11 Polyclonal antibody (10316-1-AP)

    WB analysis using 10316-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10316-1-AP (WBP11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using WBP11 Polyclonal antibody (10316-1-AP)

    IP experiment of mouse heart using 10316-1-AP

    IP result of anti-WBP11 (IP:10316-1-AP, 3ug; Detection:10316-1-AP 1:500) with mouse heart tissue lysate 6000ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using WBP11 Polyclonal antibody (10316-1-AP)

    IHC staining of human breast cancer using 10316-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10316-1-AP (WBP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using WBP11 Polyclonal antibody (10316-1-AP)

    IHC staining of human breast cancer using 10316-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10316-1-AP (WBP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using WBP11 Polyclonal antibody (10316-1-AP)

    IF Staining of HeLa using 10316-1-AP

    Immunofluorescent analysis of HeLa cells using 10316-1-AP (WBP11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, mouse heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10316-1-AP targets WBP11 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WBP11 fusion protein Ag0366 相同性解析による交差性が予測される生物種
Full Name WW domain binding protein 11
Calculated molecular weight 70 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC001621
Gene symbol WBP11
Gene ID (NCBI) 51729
RRIDAB_10694664
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WBP11 (WW domain-binding protein 11) is a protein that in humans, is encoded by the WBP11 gene. The function of WBP11 is to play a role in the regulation of pre-mRNA processing. More specifically, this nuclear protein, colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. The predicted MW of this protein is 70 kDa, and this antibody specially recognises the 70 kDa protein.

Protocols

Product Specific Protocols
WB protocol for WBP11 antibody 10316-1-APDownload protocol
IHC protocol for WBP11 antibody 10316-1-APDownload protocol
IF protocol for WBP11 antibody 10316-1-APDownload protocol
IP protocol for WBP11 antibody 10316-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols