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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Bcl-XL Monoclonal antibody, PBS Only (Detector)

Bcl-XL Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4C12A6

Cat no : 66020-1-PBS

Synonyms

BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, Apoptosis regulator Bcl X

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Bcl-XL Monoclonal antibody (66020-1-Ig)

    WB analysis of Jurkat using 66020-1-Ig (same clone as 66020-1-PBS)

    Jurkat, Ramos, K-562 and Raw 264.7 cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using Bcl-XL Monoclonal antibody (66020-1-Ig)

    WB analysis of A431 using 66020-1-Ig (same clone as 66020-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Bcl-XL Monoclonal antibody (66020-1-Ig)

    WB analysis of HeLa using 66020-1-Ig (same clone as 66020-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using Bcl-XL Monoclonal antibody (66020-1-Ig)

    WB analysis of Jurkat using 66020-1-Ig (same clone as 66020-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Bcl-XL Monoclonal antibody (66020-1-Ig)

    IHC staining of human breast cancer using 66020-1-Ig (same clone as 66020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Bcl-XL Monoclonal antibody (66020-1-Ig)

    IHC staining of human breast cancer using 66020-1-Ig (same clone as 66020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Bcl-XL Monoclonal antibody (66020-1-Ig)

    IHC staining of human lymphoma using 66020-1-Ig (same clone as 66020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Bcl-XL Monoclonal antibody (66020-1-Ig)

    IHC staining of human testis using 66020-1-Ig (same clone as 66020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Bcl-XL Monoclonal antibody (66020-1-Ig)

    IHC staining of human testis using 66020-1-Ig (same clone as 66020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Bcl-XL Monoclonal antibody (66020-1-Ig)

    IHC staining of human lymphoma using 66020-1-Ig (same clone as 66020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Bcl-XL Monoclonal antibody (66020-1-Ig)

    IHC staining of human colon using 66020-1-Ig (same clone as 66020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Bcl-XL Monoclonal antibody (66020-1-Ig)

    IHC staining of human colon using 66020-1-Ig (same clone as 66020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Bcl-XL Monoclonal antibody (66020-1-Ig)

    IF Staining of HepG2 using 66020-1-Ig (same clone as 66020-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Bcl-XL antibody (66020-1-Ig, Clone: 4C12A6 ) at dilution of 1:1355 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Bcl-XL Monoclonal antibody (66020-1-Ig)

    IF Staining of HeLa using 66020-1-Ig (same clone as 66020-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Bcl-XL antibody (66020-1-Ig, Clone: 4C12A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Bcl-XL Monoclonal antibody (66020-1-Ig)

    FC experiment of HeLa using 66020-1-Ig (same clone as 66020-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Bcl-XL (66020-1-Ig, Clone:4C12A6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50613-1

    Cytometric bead array standard curve of MP50613-1

    Cytometric bead array standard curve of MP50613-1, Bcl-xL Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66020-2-PBS. Detection antibody: 66020-1-PBS. Standard:Ag18037. Range: 1.563-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66020-1-PBS targets Bcl-XL as part of a matched antibody pair:

MP50613-1: 66020-2-PBS capture and 66020-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Bcl-XL fusion protein Ag18037 相同性解析による交差性が予測される生物種
Full Name BCL2-like 1
Calculated molecular weight 26 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC019307
Gene symbol Bcl-XL
Gene ID (NCBI) 598
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.