• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HDAC1 Recombinant antibody, PBS Only (Capture)

HDAC1 Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240614A3

Cat no : 83624-1-PBS

Synonyms

histone deacetylase 1, HD1, GON 10, EC:3.5.1.98, EC:3.5.1.-

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using HDAC1 Recombinant antibody (83624-1-RR)

    WB analysis using 83624-1-RR (same clone as 83624-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83624-1-RR (HDAC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83624-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HDAC1 Recombinant antibody (83624-1-RR)

    IF Staining of HeLa using 83624-1-RR (same clone as 83624-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HDAC1 antibody (83624-1-RR, Clone: 240614A3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83624-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HDAC1 Recombinant antibody (83624-1-RR)

    IF Staining of HeLa using 83624-1-RR (same clone as 83624-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HDAC1 antibody (83624-1-RR, Clone: 240614A3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83624-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using HDAC1 Recombinant antibody (83624-1-RR)

    FC experiment of HeLa using 83624-1-RR (same clone as 83624-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HDAC1 Recombinant antibody (83624-1-RR, Clone:240614A3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83624-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00577-3

    Cytometric bead array standard curve of MP00577-3

    Cytometric bead array standard curve of MP00577-3, HDAC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83624-1-PBS. Detection antibody: 83624-3-PBS. Standard: Ag0256. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83624-1-RR

    Affinity and Kinetic Characterization of 83624-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83624-1-RR against Human HDAC1 were performed. The affinity constant is below 1 pM.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83624-1-PBS targets HDAC1 as part of a matched antibody pair:

MP00577-3: 83624-1-PBS capture and 83624-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HDAC1 fusion protein Ag0256 相同性解析による交差性が予測される生物種
Full Name histone deacetylase 1
Calculated molecular weight 55 kDa
GenBank accession numberBC000301
Gene symbol HDAC1
Gene ID (NCBI) 3065
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.