• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human beta NGF protein

Activity

0.5-3.0 ng/mL EC50

Specific Activity

>5.00 x 105 IU/mg

Species Reactivity

human

Purity

>95%

Cat no : HZ-1222

Synonyms

Beta nerve growth factor, Beta NGF, HSAN5, NGF, NGFB, proNGF



Technical Specifications

GeneID 4803
Species Human
Expression HEK293
Activity 0.5-3.0 ng/mL EC50
Specific Activity>5.00 x 105 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP01138
Molecular Mass 13 kDa reduced, 14 kDa non-reduced, non-disulfide bonded homodimer, non-glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Beta nerve growth factor (NGF) is critical for the survival and maintenance of sympathetic and sensory neurons and may play an important role in the regulation of the immune system (PMID 16842161). The presence of beta NGF in immune cells, endocrine cells, and the CNS limbic areas suggests that beta NGF may function as an intracellular messenger to regulate the body’s response to stress (PMID 19442684). Xeno-free Recombinant Human beta NGF is expressed in human 293 cells as a non-disulfide bonded homodimeric protein with an apparent molecular mass of 13 kDa. This product is produced in a human cell expression system with serum-free, chemically defined media.

Synonyms

Beta nerve growth factor, Beta NGF, HSAN5, NGF, NGFB, proNGF