• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human PDGFbb protein

Activity

0.3-3 ng/mL EC50

Specific Activity

>5.00 x 106 IU/mg

Species Reactivity

human,mouse,rat

Purity

>95%

Cat no : HZ-1308

Synonyms

Becaplermin, c sis, FLJ12858, PDGF 2, PDGF subunit B, PDGF2, PDGFB, PDGFbb, Proto oncogene c Sis, SIS, SSV



Technical Specifications

GeneID 5155
Species Human
Expression HEK293
Activity 0.3-3 ng/mL EC50
Specific Activity>5.00 x 106 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP31240
Molecular Mass 14 and 36 kDa reduced, 29 to 32 kDa non-reduced, homodimer, glycosylated
Formulation 50mM Acetate pH 3.5 + 500mM NaCl, See Certificate of Analysis for details
Species Reactivity human,mouse,rat

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

PDGFbb is a member of the platelet-derived growth family factor. Four members of this family are mitogenic factors for cells of mesenchymal origin, and they are characterized by a motif of eight cysteines. PDGFbb is required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, and placenta among others. PDGFbb plays a role in blood vessel development. It also implements its role in wound healing (PMID: 29266203; 28851707; 28556368).

Synonyms

Becaplermin, c sis, FLJ12858, PDGF 2, PDGF subunit B, PDGF2, PDGFB, PDGFbb, Proto oncogene c Sis, SIS, SSV

Publications

SpeciesTitle
human

Br J Pharmacol

A novel HSF1 activator ameliorates nonalcoholic steatohepatitis by stimulating mitochondrial adaptive oxidation

Authors - Yong Rao
mouse

J Neuroinflammation

SU16f inhibits fibrotic scar formation and facilitates axon regeneration and locomotor function recovery after spinal cord injury by blocking the PDGFRβ pathway.

Authors - Ziyu Li
mouse

Front Pharmacol

Deletion of Smooth Muscle Lethal Giant Larvae 1 Promotes Neointimal Hyperplasia in Mice.

Authors - Ya Zhang
human

Arch Biochem Biophys

Quiescin/sulfhydryl oxidase 1b (QSOX1b) induces migration and proliferation of vascular smooth muscle cells by distinct redox pathways.

Authors - Karime C França
human

Commun Biol

KLF4-PFKFB3-driven glycolysis is essential for phenotypic switching of vascular smooth muscle cells

Authors - Xinhua Zhang

FASEB J

Deficiency of thrombospondin-2 alleviates intimal hyperplasia in mice by modulating vascular smooth muscle cell proliferation and migration

Authors - Xu Li