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  • Featured Product
  • KD/KO Validated

RCAS1 Polyclonal antibody

RCAS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12255-1-AP

Synonyms

EBAG9, PDAF, Estrogen receptor-binding fragment-associated gene 9 protein, EBAG 9, EB9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RCAS1 Polyclonal antibody (12255-1-AP)

    WB analysis using 12255-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12255-1-AP (RCAS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RCAS1 Polyclonal antibody (12255-1-AP)

    WB analysis of HEK-293 using 12255-1-AP

    WB result of RCAS1 antibody (12255-1-AP, 1:10000) with si-control and si-RCAS1 transfected HEK293 cells.

  • Immunoprecipitation (IP) experiment of HeLa cells using RCAS1 Polyclonal antibody (12255-1-AP)

    IP experiment of HeLa using 12255-1-AP

    IP result of anti-RCAS1 (IP:12255-1-AP, 4ug; Detection:12255-1-AP 1:2000) with HeLa cells lysate 1720 ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using RCAS1 Polyclonal antibody (12255-1-AP)

    IHC staining of human colon using 12255-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12255-1-AP (RCAS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using RCAS1 Polyclonal antibody (12255-1-AP)

    IHC staining of human colon using 12255-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12255-1-AP (RCAS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using RCAS1 Polyclonal antibody (12255-1-AP)

    IF Staining of HEK-293 using 12255-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using RCAS1 antibody (12255-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse heart tissue, rat heart tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12255-1-AP targets RCAS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RCAS1 fusion protein Ag2905 相同性解析による交差性が予測される生物種
Full Name estrogen receptor binding site associated, antigen, 9
Calculated molecular weight 213 aa, 24 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC017729
Gene symbol RCAS1
Gene ID (NCBI) 9166
RRIDAB_2246420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Estrogen receptor-binding fragment-associated antigen 9 (EBAG9) gene was identified as an estrogen-responsive gene. The gene product, receptor-binding cancer antigen expressed on SiSo cells (RCAS1), is associated with aggressive characteristics and poor overall survival for 15 different human malignancies. The correlation between RCAS1 expression and several clinicopathological variables, including tumor size, clinical stage, invasion depth and lymph node metastasis highlights this molecule's clinical significance. Expression of RCAS1 in tumor cells plays an important role in evasion from host immune system resulting tumor progression, invasion and metastasis. Further exploration of RCAS1 biological function will facilitate development of novel therapeutic strategies that target RCAS1.

Protocols

Product Specific Protocols
WB protocol for RCAS1 antibody 12255-1-APDownload protocol
IHC protocol for RCAS1 antibody 12255-1-APDownload protocol
IF protocol for RCAS1 antibody 12255-1-APDownload protocol
IP protocol for RCAS1 antibody 12255-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IHC

J Ethnopharmacol

Exploring the inhibitory impact of Mongolian medicinal He-Zi-3 soup on mammary gland hyperplasia in rats induced by estrogen and progestogen

Authors - Chunlan Liang
View Article