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KD/KO Validated

EGFR-Specific Polyclonal antibody

Name: EGFR-Specific Polyclonal antibody
Brand: Proteintech
SKU: 18986-1-AP
Rating: 4.40 (5 reviews)

EGFR-Specific Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

154

Reviews (5)

Cat no : 18986-1-AP

Synonyms

EGFR, EGFR-Specific, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1

Validation Data Gallery

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AllWBIP

WB analysis using 18986-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 18986-1-AP
WB result of EGFR-Specific antibody (18986-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EGFR-Specific transfected HepG2 cells.
WB analysis of HeLa/RPE1 cells using 18986-1-AP
WB result ofanti-EGFR (18986-1-AP) in Hela and REP1 cell by Dr.Kodani, Andrew.
WB analysis using 18986-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 18986-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 18986-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of NCI-H1299 using 18986-1-AP
NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 18986-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
IP experiment of MCF-7 using 18986-1-AP
IP result of anti-EGFR-Specific (IP:18986-1-AP, 3ug; Detection:18986-1-AP 1:500) with MCF-7 cells lysate 2500ug.

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Cat No. 18986-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, MCF-7 cells, A431 cells, HeLa/RPE1 cells, NCI-H1299 cells, HeLa cells, A549 cells, L02 cells, NCL-H1299 cells
Positive IP detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 131 publications below
IHC	See 21 publications below
IF	See 19 publications below
IP	See 9 publications below
FC	See 1 publications below
CoIP	See 6 publications below
ChIP	See 1 publications below

Product Information

18986-1-AP targets EGFR-Specific in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat, pig, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Calculated molecular weight	134 kDa
Observed molecular weight	145-165 kDa
GenBank accession number	NM_005228
Gene symbol	EGFR
Gene ID (NCBI)	1956
RRID	AB_10596476
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ERBB1, also named as EGFR, is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer. The antibody is specific to isoform1.

Protocols

Product Specific Protocols
WB protocol for EGFR-Specific antibody 18986-1-AP	Download protocol
IP protocol for EGFR-Specific antibody 18986-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Nat Microbiol Interferon-induced transmembrane protein-1 competitively blocks Ephrin receptor A2-mediated Epstein-Barr virus entry into epithelial cells Authors - Yinggui Yang View Article
mouse	WB,IHC	Cell Res In vivo self-assembled small RNAs as a new generation of RNAi therapeutics. Authors - Zheng Fu KD Validated View Article
human	WB	Cancer Commun (Lond) Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma Authors - Xiaoteng Cui View Article
human	IF	ACS Appl Mater Interfaces On-Chip Replication of Extremely Early-Stage Tumor Behavior. Authors - Chengpan Li View Article
pig	WB	Biosens Bioelectron Microneedle patches integrated with lateral flow cassettes for blood-free chronic kidney disease point-of-care testing during a pandemic. Authors - Yi-Jyun Chen View Article
human	WB, IHC, ChIP	Cancer Res Hypoxia Alters the Response to Anti-EGFR Therapy by Regulating EGFR Expression and Downstream Signaling in a DNA Methylation-Specific and HIF-Dependent Manner. Authors - Mahelet Mamo View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Marina (Verified Customer) (02-13-2023)	A stronger band can be observed slighly above 170 kDa and a fainter band below 130 kDa. Ponceau red is used as total protein loading control. Primary antibody dilution 1:500, overnight incubation at 4ºC. Exposure time 3 minutes. Applications: Western Blot Primary Antibody Dilution: 1:500 Cell Tissue Type: Human triple negative breast cancer cells. EGFR-Specific Antibody Western Blot validation (1:500 dilution) in Human triple negative breast cancer cells. (Cat no:18986-1-AP)
FH Clarisse (Verified Customer) (12-12-2022)	This antibody works very well on tissue fixed overnight at 4 degrees C with 2% PFA. No antigen retrieval required. Applications: Immunofluorescence Primary Antibody Dilution: 1:1000 Cell Tissue Type: Human cerebral organoids
FH Morgan (Verified Customer) (10-26-2021)	I had luck with this antibody as seen from the image attached. It took a little bit of time to develop though. Maybe using more than 1:500 would be better. Unfortunately I couldn't get the phosphorylated version (p-EGF) to work though. I used OV90 (ovarian cancer) cells Applications: Western Blot Primary Antibody Dilution: 1:500 Cell Tissue Type: OV90 ovarian cancer cells EGFR-Specific Antibody Western Blot validation (1:500 dilution) in OV90 ovarian cancer cells (Cat no:18986-1-AP)
FH K (Verified Customer) (12-20-2020)	worked well for WB (1:1000)IF (1:200)and IHC (1:200) Applications: Western Blot, Immunohistochemistry, Immunofluorescence Cell Tissue Type: mouse liver tissues. rat liver and human liver cells EGFR-Specific Antibody Western Blot,Immunohistochemistry,Immunofluorescence validation ( dilution) in mouse liver tissues. rat liver and human liver cells (Cat no:18986-1-AP)
FH XB (Verified Customer) (10-29-2020)	It is OK to use it to probe EGFR (about 180 kD), but there are some unspecific bands at the lower part of the gel. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: PC12 cell EGFR-Specific Antibody Western Blot validation (1:1000 dilution) in PC12 cell (Cat no:18986-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EGFR-Specific Polyclonal antibody

EGFR-Specific Polyclonal Antibody for WB, IP, ELISA

Validation Data Gallery

WB analysis using 18986-1-AP

WB analysis of HepG2 using 18986-1-AP

WB analysis of HeLa/RPE1 cells using 18986-1-AP

WB analysis using 18986-1-AP

WB analysis using 18986-1-AP

WB analysis of MCF-7 using 18986-1-AP

WB analysis of NCI-H1299 using 18986-1-AP

WB analysis of A431 using 18986-1-AP

IP experiment of MCF-7 using 18986-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Interferon-induced transmembrane protein-1 competitively blocks Ephrin receptor A2-mediated Epstein-Barr virus entry into epithelial cells

In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.

Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma

On-Chip Replication of Extremely Early-Stage Tumor Behavior.

Microneedle patches integrated with lateral flow cassettes for blood-free chronic kidney disease point-of-care testing during a pandemic.

Hypoxia Alters the Response to Anti-EGFR Therapy by Regulating EGFR Expression and Downstream Signaling in a DNA Methylation-Specific and HIF-Dependent Manner.

Reviews

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