Validation Data Gallery
Tested Applications
| Positive WB detected in | recombinant protein, Transfected HEK-293 cells |
| Positive IP detected in | Transfected HEK-293T cells |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
50430-2-AP targets GFP tag in WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, RIP, IP-MS, ELISA applications and shows reactivity with aequorea victoria, recombinant protein samples.
| Tested Reactivity | aequorea victoria, recombinant protein |
| Cited Reactivity | mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GFP tag fusion protein Ag2128 相同性解析による交差性が予測される生物種 |
| Full Name | GFP tag |
| Calculated molecular weight | 26 kDa |
| GenBank accession number | M62653 |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_11042881 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages.
Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.
When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.
This antibody is a rabbit polyclonal antibody, generated against the full-length eGFP protein. It exhibits reactivity towards variants of Aequorea victoria GFP, including S65T-GFP, RS-GFP, YFP, CFP, and eGFP.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GFP tag antibody 50430-2-AP | Download protocol |
| IF protocol for GFP tag antibody 50430-2-AP | Download protocol |
| IP protocol for GFP tag antibody 50430-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis | ||
Gastroenterology PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis | ||
Nat Genet Pathogenic SPTBN1 variants cause an autosomal dominant neurodevelopmental syndrome. | ||
Mol Plant A TT1-SCE1 module integrates ubiquitination and SUMOylation to regulate heat tolerance in rice |