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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ATP5F1 Polyclonal antibody

ATP5F1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15999

Synonyms

ATP5F1, ATPase subunit b, PIG47

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL488-15999

    FC experiment of HeLa using CL488-15999

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ATP5F1 (CL488-15999) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 15999-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-15999 targets ATP5F1 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP5F1 fusion protein Ag8571 相同性解析による交差性が予測される生物種
Full Name ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1
Calculated molecular weight 256 aa, 29 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC005366
Gene symbol ATP5F1
Gene ID (NCBI) 515
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP5F1(ATP synthase subunit b) belongs to the eukaryotic ATPase B chain family. The ATP5F1 gene encodes subunit B of the mitochondrial ATP synthase Fo unit, which contains 214-amino acid with a 42-amino acid import signal(PMID:1831354). Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 ATP5F1 antibody CL488-15999Download protocol
Standard Protocols
Click here to view our Standard Protocols