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KD/KO Validated

CPSF3 Polyclonal antibody

Name: CPSF3 Polyclonal antibody
Brand: Proteintech
SKU: 11609-1-AP

CPSF3 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11609-1-AP

Synonyms

CPSF, CPSF 73, CPSF 73 kDa subunit, CPSF3, CPSF73, YSH1

Validation Data Gallery

Applications:

AllWBIP

WB analysis of HEK-293 using 11609-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human skin using 11609-1-AP
human skin tissue were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11609-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11609-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11609-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 11609-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:700.

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Cat No. 11609-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HepG2 cells, human brain tissue, human skin tissue
Positive IP detected in	HEK-293 cells

Planning an IHC experiment? We recommend our IHCeasy CPSF3 Ready-To-Use IHC Kit. CPSF3 primary antibody included.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below

Product Information

11609-1-AP targets CPSF3 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CPSF3 fusion protein Ag2173 相同性解析による交差性が予測される生物種
Full Name	cleavage and polyadenylation specific factor 3, 73kDa
Calculated molecular weight	684 aa, 77 kDa
Observed molecular weight	77 kDa
GenBank accession number	BC020211
Gene symbol	CPSF3
Gene ID (NCBI)	51692
RRID	AB_2292188
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for CPSF3 antibody 11609-1-AP	Download protocol
IP protocol for CPSF3 antibody 11609-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res LncRNA CASC9 interacts with CPSF3 to regulate TGF-β signaling in colorectal cancer. Authors - Kaili Luo KD Validated View Article
human	WB	Cancers (Basel) CPSF3 Promotes Pre-mRNA Splicing and Prevents CircRNA Cyclization in Hepatocellular Carcinoma Authors - Ying Huang View Article
human	WB	Cell Rep Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome. Authors - J J David Ho View Article
pig	WB	Cell Mol Life Sci Single-cell multi-omics profiling reveals key regulatory mechanisms that poise germinal vesicle oocytes for maturation in pigs Authors - Xiaolong Yuan View Article
human	WB	Cell Chem Biol Anticancer benzoxaboroles block pre-mRNA processing by directly inhibiting CPSF3 Authors - Ye Tao View Article

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CPSF3 Polyclonal antibody

CPSF3 Polyclonal Antibody for IP, WB, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 11609-1-AP

WB analysis of human skin using 11609-1-AP

WB analysis of human brain using 11609-1-AP

WB analysis of HepG2 using 11609-1-AP

WB analysis of HEK-293 using 11609-1-AP

IP experiment of HEK-293 using 11609-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

LncRNA CASC9 interacts with CPSF3 to regulate TGF-β signaling in colorectal cancer.

CPSF3 Promotes Pre-mRNA Splicing and Prevents CircRNA Cyclization in Hepatocellular Carcinoma

Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome.

Single-cell multi-omics profiling reveals key regulatory mechanisms that poise germinal vesicle oocytes for maturation in pigs

Anticancer benzoxaboroles block pre-mRNA processing by directly inhibiting CPSF3