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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CXCL12/SDF-1 Polyclonal antibody

CXCL12/SDF-1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17402

Synonyms

C X C motif chemokine 12, CXCL12, hIRH, hSDF 1, IRH, PBSF, SCYB12, SDF 1, SDF 1a, SDF 1b, SDF1, SDF1A, SDF1B, Stromal cell derived factor 1, TLSF a, TLSF b, TPAR1

Validation Data Gallery

Applications:
  • FC experiment of Jurkat using CL488-17402

    FC experiment of Jurkat using CL488-17402

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CXCL12/SDF-1 (CL488-17402) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 17402-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-17402 targets CXCL12/SDF-1 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXCL12/SDF-1 fusion protein Ag11379 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)
Calculated molecular weight 89 aa, 10 kDa
GenBank accession numberBC039893
Gene symbol CXCL12
Gene ID (NCBI) 6387
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

In adulthood, CXCL12 plays an important role in angiogenesis by recruiting endothelial progenitor cells (EPCs) from the bone marrow through a CXCR4 dependent mechanism (PMID: 17878755). It is this function of CXCL12 that makes it a very important factor in carcinogenesis and the neovascularisation linked to tumour progression (PMID: 16943240). CXCL12 also has a role in tumor metastasis where cancer cells that express the receptor CXCR4 are attracted to metastasis target tissues that release the ligand, CXCL12 (PMID: 11242036 ). In breast cancer, however, increased expression of CXCL12 determines a reduced risk of distant metastasis (PMID: 19646861; 17724466 ). This antibody can detect all the isoforms of CXCL12/SDF1.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CXCL12/SDF-1 antibody CL488-17402Download protocol
Standard Protocols
Click here to view our Standard Protocols