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Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CXCR3B-specific Monoclonal antibody

CXCR3B-specific Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B2D6

Cat no : CL594-60065

Synonyms

CD183, CKR L2, CMKAR3, CXC R3, CXCR 3, CXCR3, CXCR3A, CXCR3B, CXCR3B-specific, G protein coupled receptor 9, GPR9, IP 10 receptor, IP10 R, Mig R, MigR

Validation Data Gallery

Applications:
  • FC experiment of U-937 using CL594-60065

    FC experiment of U-937 using CL594-60065

    1X10^6 U-937 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human CXCR3B-specific (CL594-60065, Clone:1B2D6) (red), or 0.8 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inU-937 cells
For other applications, we recommend the unconjugated version of this antibody, 60065-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-60065 targets CXCR3B-specific in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name chemokine (C-X-C motif) receptor 3
Calculated molecular weight 46 kDa
Observed molecular weight 40 kDa+43 kDa
GenBank accession numberAF469635
Gene symbol CXCR3
Gene ID (NCBI) 2833
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CXCR3 (C-X-C chemokine receptor type 3) is a G protein-coupled, seven-transmembrane domain chemokine receptor that is expressed on the surface of a number of cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells (PMID: 16847335). CXCR3 binds and is activated by the three IFN-gamma-inducible chemokines: CXCL9, CXCL10, and CXCL11. Binding of these ligands to CXCR3 has been implicated in regulating T-cell infiltration during inflammation and tissue injury (PMID: 17250586). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms, CXCR3B, mediates the angiostatic activity of CXCR3 ligands and also acts as functional receptor for CXCL4 (PMID:12782716). This monoclonal antibody is specific to CXCR3B.

Protocols

Product Specific Protocols
FC protocol for CL594 CXCR3B-specific antibody CL594-60065Download protocol
Standard Protocols
Click here to view our Standard Protocols