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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EEF1A1 Recombinant antibody, PBS Only

EEF1A1 Recombinant Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B16

Cat no : 81377-1-PBS

Synonyms

CCS 3, CCS3, EEF 1, EEF1A, eEF1A 1, EEF1A1, EF 1 alpha 1, EF Tu, EF1A, Elongation factor 1 alpha 1, Elongation factor Tu, FLJ25721, GRAF 1EF, HNGC:16303, LENG7, PTI1

Validation Data Gallery

Applications:
  • WB analysis using 81377-1-RR (same clone as 81377-1-PBS)

    WB analysis using 81377-1-RR (same clone as 81377-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81377-1-RR (EEF1A1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81377-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 81377-1-RR (same clone as 81377-1-PBS)

    IP experiment of HeLa using 81377-1-RR (same clone as 81377-1-PBS)

    IP result of anti-EEF1A1 (IP:81377-1-RR, 4ug; Detection:81377-1-RR 1:8000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 81377-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 81377-1-RR (same clone as 81377-1-PBS)

    IHC staining of human pancreas cancer using 81377-1-RR (same clone as 81377-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81377-1-RR (EEF1A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81377-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF1A1 antibody included
  • IHC staining of human pancreas cancer using 81377-1-RR (same clone as 81377-1-PBS)

    IHC staining of human pancreas cancer using 81377-1-RR (same clone as 81377-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81377-1-RR (EEF1A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81377-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF1A1 antibody included
  • IHC staining of mouse brain using 81377-1-RR (same clone as 81377-1-PBS)

    IHC staining of mouse brain using 81377-1-RR (same clone as 81377-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81377-1-RR (EEF1A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81377-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF1A1 antibody included
  • IHC staining of mouse brain using 81377-1-RR (same clone as 81377-1-PBS)

    IHC staining of mouse brain using 81377-1-RR (same clone as 81377-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81377-1-RR (EEF1A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81377-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF1A1 antibody included
  • IF Staining of HepG2 using 81377-1-RR (same clone as 81377-1-PBS)

    IF Staining of HepG2 using 81377-1-RR (same clone as 81377-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EEF1A1 antibody (81377-1-RR, Clone: 2B16 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81377-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 81377-1-RR (same clone as 81377-1-PBS)

    IF Staining of HepG2 using 81377-1-RR (same clone as 81377-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using EEF1A1 antibody (81377-1-RR, Clone: 2B16 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81377-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy EEF1A1 Ready-To-Use IHC Kit. EEF1A1 primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

81377-1-PBS targets EEF1A1 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EEF1A1 fusion protein Ag1938 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation elongation factor 1 alpha 1
Calculated molecular weight 462 aa, 50 kDa
Observed molecular weight50 kDa
GenBank accession numberBC014224
Gene symbol EEF1A1
Gene ID (NCBI) 1915
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

EEF1A1, also named as EEF1A, EF1A and LENG7, belongs to the GTP-binding elongation factor family. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It is a typical housekeeping gene product required for the maintenance of cell proliferation and/or survival. In addition, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved.