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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated EPCAM Polyclonal antibody

EPCAM Polyclonal Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-21050

Synonyms

CD326, CO 17A, CO17 1A, EGP, EGP 2, EGP314, EGP34, EGP40, Ep CAM, EPCAM, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733 2, hEGP 2, hEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, M4S1, MIC18, MK 1, TACST 1, TACSTD1, TROP1

Validation Data Gallery

Applications:
  • FC experiment of MCF-7 using CL555-21050

    FC experiment of MCF-7 using CL555-21050

    1X10^6 MCF-7 cells were surface stained with 0.4 ug CoraLite®555 Anti-Human EPCAM (CL555-21050) (red), or 0.4 ug Control Antibody. Cells were not fixed.

Tested Applications

Positive FC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 21050-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL555-21050 is EPCAM Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPCAM fusion protein Ag15393 相同性解析による交差性が予測される生物種
Full Name epithelial cell adhesion molecule
Calculated molecular weight 314 aa, 35 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC014785
Gene symbol EPCAM
Gene ID (NCBI) 4072
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

Protocols

Product Specific Protocols
FC protocol for CL555 EPCAM antibody CL555-21050Download protocol
Standard Protocols
Click here to view our Standard Protocols