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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Ferritin light chain Monoclonal antibody, PBS Only

Ferritin light chain Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F9F5

Cat no : 68068-1-PBS

Synonyms

Ferritin L subunit, Ferritin light chain, ferritin, light polypeptide, FTL

Validation Data Gallery

Applications:
  • WB analysis of A549 using 68068-1-Ig (same clone as 68068-1-PBS)

    WB analysis of A549 using 68068-1-Ig (same clone as 68068-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68068-1-Ig (same clone as 68068-1-PBS)

    WB analysis of HeLa using 68068-1-Ig (same clone as 68068-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 68068-1-Ig (same clone as 68068-1-PBS)

    WB analysis of HepG2 using 68068-1-Ig (same clone as 68068-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 68068-1-Ig (same clone as 68068-1-PBS)

    WB analysis of human placenta using 68068-1-Ig (same clone as 68068-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 68068-1-Ig (same clone as 68068-1-PBS)

    IHC staining of mouse brain using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FTL antibody included
  • IHC staining of mouse brain using 68068-1-Ig (same clone as 68068-1-PBS)

    IHC staining of mouse brain using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FTL antibody included
  • IHC staining of human colon cancer using 68068-1-Ig (same clone as 68068-1-PBS)

    IHC staining of human colon cancer using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FTL antibody included
  • IHC staining of human colon cancer using 68068-1-Ig (same clone as 68068-1-PBS)

    IHC staining of human colon cancer using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FTL antibody included
  • IHC staining of human prostate cancer using 68068-1-Ig (same clone as 68068-1-PBS)

    IHC staining of human prostate cancer using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FTL antibody included
  • IHC staining of human prostate cancer using 68068-1-Ig (same clone as 68068-1-PBS)

    IHC staining of human prostate cancer using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FTL antibody included
  • IHC staining of human liver cancer using 68068-1-Ig (same clone as 68068-1-PBS)

    IHC staining of human liver cancer using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FTL antibody included
  • IHC staining of human liver cancer using 68068-1-Ig (same clone as 68068-1-PBS)

    IHC staining of human liver cancer using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FTL antibody included
  • IF Staining of HepG2 using 68068-1-Ig (same clone as 68068-1-PBS)

    IF Staining of HepG2 using 68068-1-Ig (same clone as 68068-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using FTL antibody (68068-1-Ig, Clone: 1F9F5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy FTL Ready-To-Use IHC Kit. FTL primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

68068-1-PBS targets Ferritin light chain in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Ferritin light chain fusion protein Ag20637 相同性解析による交差性が予測される生物種
Full Name ferritin, light polypeptide
Calculated molecular weight 19 kDa
Observed molecular weight18 kDa
GenBank accession numberBC008439
Gene symbol FTL
Gene ID (NCBI) 2512
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.