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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Glucocorticoid receptor Polyclonal antibody

Glucocorticoid receptor Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-24050

Synonyms

GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1

Validation Data Gallery

Applications:
  • IF Staining of A549 using CL488-24050

    IF Staining of A549 using CL488-24050

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 Glucocorticoid receptor antibody (CL488-24050) at dilution of 1:200.

  • FC experiment of HeLa using CL488-24050

    FC experiment of HeLa using CL488-24050

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Glucocorticoid receptor (CL488-24050) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inA549 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NR3C1 Ready-To-Use IHC Kit. NR3C1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 24050-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

The immunogen of CL488-24050 is Glucocorticoid receptor Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Glucocorticoid receptor fusion protein Ag21146 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Calculated molecular weight 86 kDa
Observed molecular weight 94-97 kDa
GenBank accession numberBC015610
Gene symbol NR3C1
Gene ID (NCBI) 2908
RRIDAB_2919198
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NR3C1 is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight [PMID:22810058].

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Glucocorticoid receptor antibody CL488-24050Download protocol
FC protocol for CL Plus 488 Glucocorticoid receptor antibody CL488-24050Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Curr Biol

Hypothalamic AgRP neurons exert top-down control on systemic TNF-α release during endotoxemia

Authors - Mehdi Boutagouga Boudjadja
View Article