• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HAO1 Polyclonal antibody

HAO1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25056-1-AP

Synonyms

Glycolate oxidase, GOX, GOX1, HAO1, HAOX1, Hydroxyacid oxidase 1

Validation Data Gallery

Applications:
  • WB analysis of mouse liver using 25056-1-AP

    WB analysis of mouse liver using 25056-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25056-1-AP (HAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of rat liver using 25056-1-AP

    WB analysis of rat liver using 25056-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 25056-1-AP (HAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25056-1-AP is HAO1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HAO1 fusion protein Ag21884 相同性解析による交差性が予測される生物種
Full Name hydroxyacid oxidase (glycolate oxidase) 1
Calculated molecular weight 370 aa, 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC113665
Gene symbol HAO1
Gene ID (NCBI) 54363
RRIDAB_2879875
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HAO1 antibody 25056-1-APDownload protocol
IHC protocol for HAO1 antibody 25056-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols