CoraLite®594-conjugated HO-1/HMOX1 Monoclonal antibody
HO-1/HMOX1 Monoclonal Antibody for IF, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, Rat, Pig
Applications
IF, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2D10A5
Cat no : CL594-66743
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | human liver tissue |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL594-66743 targets HO-1/HMOX1 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | HO-1/HMOX1 fusion protein Ag21296 相同性解析による交差性が予測される生物種 |
| Full Name | heme oxygenase (decycling) 1 |
| Calculated molecular weight | 33 kDa |
| Observed molecular weight | 33 kDa |
| GenBank accession number | BC001491 |
| Gene symbol | HMOX1 |
| Gene ID (NCBI) | 3162 |
| RRID | AB_2883623 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 HO-1/HMOX1 antibody CL594-66743 | Download protocol |
| FC protocol for CL594 HO-1/HMOX1 antibody CL594-66743 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
