MultiProTM-5CFLX Anti-Human CD3 (OKT3)
CD3 Monoclonal Antibody for
Host / Isotype
Mouse / IgG2a, kappa
Reactivity
Human
Applications
Conjugate
5CFLX Fluorescent Dye
CloneNo.
OKT3
Cat no : G65133-1-5C
Synonyms
Validation Data Gallery
Tested Applications
Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product |
Recommended dilution
Application | Dilution |
---|---|
SINGLE CELL (INTRA) | SINGLE CELL (INTRA) : <0.5ug/test |
Sample-dependent, check data in validation data gallery |
Product Information
G65133-1-5C targets CD3 in applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2a, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | Human T cells 相同性解析による交差性が予測される生物種 |
Full Name | CD3e molecule, epsilon (CD3-TCR complex) |
Calculated molecular weight | 207 aa, 23 kDa |
GenBank accession number | BC049847 |
Gene symbol | CD3E |
Gene ID (NCBI) | 916 |
Conjugate | 5CFLX Fluorescent Dye |
Form | Liquid |
Purification Method | |
Storage Buffer | PBS with 1mM EDTA and 0.09% sodium azide |
Storage Conditions | 2-8°C |
Background Information
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).