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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PRKCSH Polyclonal antibody

PRKCSH Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12148

Synonyms

80K H protein, AGE R2, G19P1, Glucosidase 2 subunit beta, Glucosidase II subunit beta, PCLD, PKCSH, PLD1, PRKCSH

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-12148

    IF Staining of HeLa using CL488-12148

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PRKCSH antibody (CL488-12148) at dilution of 1:200.

Tested Applications

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PRKCSH Ready-To-Use IHC Kit. PRKCSH primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12148-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-12148 targets PRKCSH in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRKCSH fusion protein Ag2796 相同性解析による交差性が予測される生物種
Full Name protein kinase C substrate 80K-H
Calculated molecular weight 80 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC013586
Gene symbol PRKCSH
Gene ID (NCBI) 5589
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRKCSH encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Defects in PRKCSH are a cause of an autosomal dominant polycystic liver disease (PCLD). Glucosidase II is an ER-localized enzyme that contains α and β subunits (Glucosidase IIα and Glucosidase IIβ) which form a defined heterodimeric complex.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PRKCSH antibody CL488-12148Download protocol
Standard Protocols
Click here to view our Standard Protocols