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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RGS7 Polyclonal antibody

RGS7 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14056-1-AP

Synonyms

RGS7

Validation Data Gallery

Applications:
  • WB analysis of SH-SY5Y using 14056-1-AP

    WB analysis of SH-SY5Y using 14056-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14056-1-AP (RGS7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 14056-1-AP

    WB analysis of SH-SY5Y using 14056-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14056-1-AP (RGS7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 14056-1-AP

    IHC staining of human gliomas using 14056-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 14056-1-AP (RGS7 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human gliomas using 14056-1-AP

    IHC staining of human gliomas using 14056-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 14056-1-AP (RGS7 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of SH-SY5Y using 14056-1-AP

    IF Staining of SH-SY5Y using 14056-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 14056-1-AP (RGS7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14056-1-AP targets RGS7 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RGS7 fusion protein Ag4982 相同性解析による交差性が予測される生物種
Full Name regulator of G-protein signaling 7
Calculated molecular weight 58 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC022009
Gene symbol RGS7
Gene ID (NCBI) 6000
RRIDAB_2238316
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RGS7 antibody 14056-1-APDownload protocol
IHC protocol for RGS7 antibody 14056-1-APDownload protocol
IF protocol for RGS7 antibody 14056-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols