SERCA2,ATP2A2 Polyclonal antibody
SERCA2,ATP2A2 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 27311-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse heart tissue, HEK-293 cells, mouse heart unboiled, 37°C incubated HEK-293 cells |
Positive IHC detected in | mouse skeletal muscle tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | C2C12 cells, mouse heart tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
Product Information
27311-1-AP targets SERCA2,ATP2A2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SERCA2,ATP2A2 fusion protein Ag26177 相同性解析による交差性が予測される生物種 |
Full Name | ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 |
Calculated molecular weight | 1042 aa, 115 kDa |
Observed molecular weight | 110-115 kDa |
GenBank accession number | BC035588 |
Gene symbol | ATP2A2 |
Gene ID (NCBI) | 488 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.
Protocols
Product Specific Protocols | |
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WB protocol for SERCA2,ATP2A2 antibody 27311-1-AP | Download protocol |
IHC protocol for SERCA2,ATP2A2 antibody 27311-1-AP | Download protocol |
IF protocol for SERCA2,ATP2A2 antibody 27311-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
JCI Insight Critical role of Znhit1 for postnatal heart function and vacuolar cardiomyopathy. |