ChromoTek V5-Trap® Magnetic Particles M-270 Kit

The ChromoTek V5-Trap® Magnetic Particles M-270 Kit consists of an anti-V5-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. V5-Trap Magnetic Particles M-270 Kit is used to immunoprecipitate V5-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.

Specificity

V5-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Particles M-270

Type

Nanobody

Cat no : v5tdk

Synonyms

V5, V5 tag, V5-tag, V5-Trap®, V5-Trap® Magnetic Particles M-270, V5-Trap® Magnetic Particles M-270 Kit, v5tdk, v5tdk-20



Product Information

The ChromoTek V5-Trap® Magnetic Particles M-270 Kit consists of an anti-V5-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. V5-Trap Magnetic Particles M-270 Kit is used to immunoprecipitate V5-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.

DescriptionThe V5-Trap Magnetic Particles M-270 Kit contains V5-Trap Magnetic Particles M-270, lysis, wash, and elution buffers for efficient immunoprecipitation of V5-tagged proteins and their interacting factors. V5-Trap Magnetic Particles M-270 is recommended when very large proteins/complexes are investigated and magnetic separation is needed for IP.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains on the SDS-PAGE

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, CoIP, ChIP, RIP, Protein purification
Specificity/TargetV5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.
Binding capacity0.62-1.25 μg of recombinant V5-tagged protein (~30 kDa) per 25 μL bead slurry
ConjugateMagnetic Particles M-270, size: 2.8 µm
high throughput-compatible
Elution bufferV5-peptide
SDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility2 % Nonidet P40 Substitute, 2 % Triton X-100, 150 mM NaCl, 10 mM TCEP, 10 mM DTT,  50 mM Urea
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 40 nM
Compatibility with mass spectrometryThe V5-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2892994
Storage BufferPBS with 0.09% sodium azide
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

Component Description
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash bufferRemoval of unwanted proteins, peptides, etc.
V5-Trap® Magnetic Particles M-27020 reactions (500 µL)