- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human EPO protein- GMP grade
EC50
0.2-3.0 ng/mL EC50
Species Reactivity
human
Purity
>95%
Cat no : HZ-1168-GMP
Synonyms
EP, EPO, Epoetin, erythropoietin, MVCD2
Validation Data Gallery
Technical Specifications
GeneID | 2056 |
Species | Human |
Expression | HEK293 |
EC50 | 0.2-3.0 ng/mL EC50 |
Purity | >95% |
Endotoxin | < 0.1 EU/µg |
Accession Number | P01588 |
Molecular Mass | 36 kDa reduced and non-reduced, monomer, glycosylated |
Formulation | 1x PBS, See Certificate of Analysis for details |
Species Reactivity | human |
Stability and Reconstitution
Stability and Storage | Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products. |
Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). |
GMP Quality Policies
HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.
Background
Erythropoietin (Epo) is a member of the EPO/TPO family. It is a secreted, glycosylated cytokine composed of four alpha helical bundles. Epo is found in the plasma and regulates erythrocyte production by promoting erythroid differentiation and initiating hemoglobin synthesis through activation of its cognate receptor (PMID 29458727). Epo also has neuroprotective activity against a variety of potential brain injuries and anti-apoptotic functions in several tissue types (PMID 11854521).
Synonyms
EP, EPO, Epoetin, erythropoietin, MVCD2