Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using TOM20 antibody (11802-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
Various lysates were subjected to SDS PAGE followed by western blot with 11802-1-AP (TOM20 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11802-1-AP (TOM20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11802-1-AP (TOM20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-TOM20 (IP:11802-1-AP, 3ug; Detection:11802-1-AP 1:2000) with HEK-293 cells lysate 2400ug.
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human TOM20 (11802-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
