Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-Beta Tubulin monoclonal antibody (Cat.NO. 66240-1-Ig, isotype IgG2a). Multi-rAb CoraLite® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM006 was used at 1:10000 for detection. Different devices were used for imaging for the same membrane.
Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-ZNF174 monoclonal antibody (Cat.NO. 68426-1-Ig, isotype IgG3). Multi-rAb CoraLite® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM006 was used at 1:10000 for detection. Different devices were used for imaging for the same membrane.
Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-EIF3E monoclonal antibody (Cat.NO. 67095-1-Ig, isotype IgG1) at dilution of 1:50000. Multi-rAb CoraLite® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM006 was used at 1:10000 for detection.
Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-Catalase monoclonal antibody (Cat.NO. 66765-1-Ig, isotype IgG1) at dilution of 1:10000. Multi-rAb CoraLite® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM006 was used at 1:10000 for detection.
Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-Beta Tubulin monoclonal antibody (Cat.NO. 66240-1-Ig, isotype IgG2a) at dilution of 1:100000. Multi-rAb CoraLite® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM006 was used at 1:10000 for detection.
Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-FAK monoclonal antibody (Cat.NO. 66258-1-Ig, isotype IgG2c) at dilution of 1:5000. Multi-rAb CoraLite® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM006 was used at 1:10000 for detection.
Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-ZNF174 monoclonal antibody (Cat.NO. 68426-1-Ig, isotype IgG3) at dilution of 1:2000. Multi-rAb CoraLite® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM006 was used at 1:10000 for detection.
Immunofluorescence of A431 cells: PFA-fixed cells were stained with anti-B23 (60096-1-Ig), followed by Multi-rAb CoraLite® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM006, 1:500, red), nuclei stained with DAPI (cyan). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich. Device setting for target: Laser Ex max 764nm, filter Em max 775nm.
Immunofluorescence of A431 cells: PFA-fixed cells were co-stained with mouse anti-B23 (60096-1-Ig) and rabbit anti-Lamin B1 (12987-1-AP), followed by Multi-rAb CoraLite® Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM006, 1:500, red) and Nano-Secondary srb2GCL555-1 (yellow). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich. Device setting for RGAM006: Laser Ex max 764nm, filter Em max 775nm.
1X10^6 MOLT4 were surface stained with 0.2 ug Anti-Human CD8 (65204-1-Ig, Clone: UCHT4) and Mouse IgG2a Isotype Control 66360-2-Ig. Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) RGAM006 was used at 1:500 for detection.
