MBP tag 抗体 3 文献

Mouse Monoclonal| カタログ番号: 66003-1-Ig |クローン番号: 4C6H4

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20,000円

(希望販売価格)

容量: 203 μg/150 μl

Choose quantity:

販売および在庫状況はコスモ・バイオ(株)にお問い合わせください。
distributor

種特異性:
recombinant protein

WB ポジティブ:
Recombinant protein

IP ポジティブ:
Recombinant protein protein

IF ポジティブ:
Transfected cells

推奨希釈倍率:
WB : 1:1000-1:8000
IP : 1:5000-1:50000
IF : N/A

製品情報


由来:
Mouse

精製方法:
Protein A purification

アイソタイプ:
IgG2a

バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.

ターゲット情報


正式名:
MBP tag

計算分子量:
40 kDa

検出分子量:
40 kDa

GenBank accession number:

Gene ID (NCBI):

遺伝子シンボル:

別名:
Maltose Binding Protein, MBP, MBP Tag
背景説明

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.


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