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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Mouse IgG2b Isotype Control (MPC-11)

IgG2b Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

MPC-11

Cat no : CL647-65128

Synonyms

CoraLite®647-conjugated Anti-Mouse Isotype Control, gamma2b, IgG2b, IgG2b Isotype Control, Igh-3, IGHG2B

Validation Data Gallery

Applications:
  • FC experiment of human peripheral blood lymphocytes using CL647-65128

    FC experiment of human peripheral blood lymphocytes using CL647-65128

    1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse IgG2b Isotype Control (CL647-65128, Clone:MPC-11) (blue) or CoraLite® Plus 647-conjugated Anti-Human CD4 (CL647-65134, clone: OKT4) (black). Cells were not fixed.

Tested Applications

Positive FC detected in
For other applications, we recommend the unconjugated version of this antibody, 65128-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65128 targets IgG2b Isotype Control in FC applications and shows reactivity with samples.

Tested Reactivity
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name immunoglobulin heavy chain 3 (serum IgG2b)
Gene symbol Igh-3
Gene ID (NCBI) 16016
Conjugate CoraLite® Plus 647 Fluorescent Dye
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The MPC-11 immunoglobulin is useful as an isotype-matched control. The MPC-11 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2b antibodies. This antibody has been quality-tested for flow cytometry as negative control.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 IgG2b Isotype Control antibody CL647-65128Download protocol
Standard Protocols
Click here to view our Standard Protocols