Resolution of the Broad Range Prestained Protein Ladder in a 4-12% Bis-Tris gel (SDS-PAGE). The image shows the migration pattern in the gel and after transfer to a PVDF membrane.
<strong>Migration patterns of Broad Range Prestained Protein Ladder in different electrophoretic conditions</strong><br>The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions. Migration patterns were determined using commercial precast mini gels.
The prestained protein ladders were resolved in an 8-16% Tris-glycine gel (SDS-PAGE). The image shows a comparison of the migration patterns on the gel for our standard (PL00001), broad (PL00002), and extra broad (PL00003) range ladders.
2 uL protein ladders were loaded in the 8%-18% Tris-glycine gel, then electrophoresed and transformed into the PVDF membrane. After blocking, this dual-channel image was taken directly by the Bio-Rad ChemiDoc MP Imaging System in the 550 nm (for 75 kDa and 310 kDa) and 680 nm (except 75 kDa and 310 kDa) range.
<strong>Migration patterns of Broad Range Prestained Protein Ladder in different electrophoretic conditions</strong><br>The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions.
Note on apparent molecular weights: Depending upon the gel type used, the coupling of a charged dye molecule to a protein marker alters the overall charge of the protein and thus its mobility in a gel. This results in differences in observed molecular weight of the protein markers between different gel types as shown in figure.
The prestained protein ladders were resolved in an 8-16% Tris-glycine gel (SDS-PAGE). The image shows a comparison of the migration patterns on the gel for our standard (PL00001), broad (PL00002), and extra broad (PL00003) range ladders.
2 uL protein ladders were loaded in the 8%-18% Tris-glycine gel, then electrophoresed and transformed into the PVDF membrane. After blocking, this dual-channel image was taken directly by the Bio-Rad ChemiDoc MP Imaging System in the 550 nm (for 75 kDa and 310 kDa) and 680 nm (except 75 kDa and 310 kDa) range.
The prestained protein ladder was resolved in a 10-20% Tris-glycine gel (SDS-PAGE). The image shows the migration pattern in the gel and after transfer to a PVDF membrane.
Note on apparent molecular weights: Depending upon the gel type used, the coupling of a charged dye molecule to a protein marker alters the overall charge of the protein and thus its mobility in a gel. This results in differences in observed molecular weight of the protein markers between different gel types.
The prestained protein ladders were resolved in an 8-16% Tris-glycine gel (SDS-PAGE). The image shows a comparison of the migration patterns on the gel for our standard (PL00001), broad (PL00002), and extra broad (PL00003) range ladders.
2 uL protein ladders were loaded in the 8%-18% Tris-glycine gel, then electrophoresed and transformed into the PVDF membrane. After blocking, this dual-channel image was taken directly by the Bio-Rad ChemiDoc MP Imaging System in the 550 nm (for 75 kDa and 310 kDa) and 680 nm (except 75 kDa and 310 kDa) range.
