The above experimental results are based on Jurkat cells cultured for three days to perform a flow cytometry experiment to detect the apoptotic cells in the cell sample.
The cell population was analyzed in THE SSC/FSC diagram:<br>FITC-A was selected on the X axis to denote CL488-AnnexinV. The Y-axis is represented by PE-A.<br><br>The meanings of the regions in the figure above:<br>Q1-UL (CL488-AnnexinV)-/PI+, cells in this region are necrotic. There may also be a small number of late apoptotic cells in it, even those with mechanical damage.<br>Q1-UR :(cl488-annexinv)+/PI+, cells in this region are late apoptotic cells.<br>Q1-LR :(cl488-annexinv)+/PI-, cells in this region are early apoptotic cells.<br>Q1-LL :(cl488-annexinv)-/PI-, cells in this region are living cells.<br><br>The apoptosis rate was usually calculated using Q1-UR+Q1-LR, late apoptosis + early apoptosis group (i.e., all AnnexinV-positive groups).
Green: Staining with CL488-Annexin V for apooptotic cells or early apoptotic cells;<br>Red: Staining with PI for dead cells;<br>Yellow: double staining with CL488-Annexin V and PI for necrotic cell or late apoptotic cells.
The above experimental results are based on Jurkat cells cultured for three days to perform a flow cytometry experiment to detect the apoptotic cells in the cell sample.
Q2-UL：Cell Group with Calcein AM -, EthD-I + (dead cells)； <br>Q2-LL：Cell Group with Calcein AM-, EthD-I - (cell debris)； <br>Q2-LR：Cell Group with Calcein AM +, EthD-I - (live cells).
Q2-UL：Cell Group with Calcein AM -, EthD-I + (dead cells)； <br>Q2-LL：Cell Group with Calcein AM-, PI - (cell debris)； <br>Q2-LR：Cell Group with Calcein AM +, E PI - (live cells).<br>
