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KD/KO Validated

ABLIM1 Polyclonal antibody

Name: ABLIM1 Polyclonal antibody
Brand: Proteintech
SKU: 15129-1-AP
Rating: 1.00 (1 reviews)

ABLIM1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 15129-1-AP

Synonyms

Actin-binding LIM protein family member 1, Actin-binding LIM protein 1, Actin-binding double zinc finger protein, actin binding LIM protein 1, abLIM-1

Validation Data Gallery

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AllWBIHCIF-PIF/ICC

WB analysis of HeLa using 15129-1-AP
WB result of ABLIM1 antibody (15129-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABLIM1 transfected HeLa cells.
WB analysis of HEK-293 using 15129-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15129-1-AP (ABLIM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NCI-H1299 using 15129-1-AP
NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 15129-1-AP (ABLIM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15129-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15129-1-AP (ABLIM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 15129-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15129-1-AP (ABLIM1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 15129-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15129-1-AP (ABLIM1 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of mouse skeletal muscle using 15129-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 15129-1-AP (ABLIM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse skeletal muscle using 15129-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 15129-1-AP (ABLIM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HEK-293 using 15129-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 15129-1-AP (ABLIM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 15129-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, NCI-H1299 cells
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse skeletal muscle tissue
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 5 publications below
IF	See 2 publications below

Product Information

15129-1-AP targets ABLIM1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ABLIM1 fusion protein Ag7258 相同性解析による交差性が予測される生物種
Full Name	actin binding LIM protein 1
Calculated molecular weight	88 kDa
Observed molecular weight	48-52 kDa, 105-110 kDa
GenBank accession number	BC002448
Gene symbol	ABLIM1
Gene ID (NCBI)	3983
RRID	AB_2257767
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ABLIM1 (actin-binding LIM protein 1) is a member of the LIM-domain protein family, mediating interactions between actin filaments and cytoplasmic targets. ABLIM1 has been reported as a cell cortex protein specifically in non-erythrocytes, and is critical for the formation of dense interwoven cortical actin meshwork. Due to the alternative splicing, ABLIM1 has multiple isoforms with predicted masses of 52, 70, and 88 kDa, respectively(PMID: 9245787). The phosphorylation or binding with SUMO2 may result in higher MW.

Protocols

Product Specific Protocols
WB protocol for ABLIM1 antibody 15129-1-AP	Download protocol
IHC protocol for ABLIM1 antibody 15129-1-AP	Download protocol
IF protocol for ABLIM1 antibody 15129-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Cell Biol miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics. Authors - Cao Jingli J KD Validated View Article
mouse	WB	J Cell Physiol Actin binding LIM 1 (abLIM1) negatively controls osteoclastogenesis by regulating cell migration and fusion. Authors - Haruna Narahara KD Validated View Article
human	WB	Int J Biol Sci Rictor promotes cell migration and actin polymerization through regulating ABLIM1 phosphorylation in Hepatocellular Carcinoma. Authors - Xin Dong KD Validated View Article
	WB	Mol Biol Cell Spatial proximity of proteins surrounding zyxin under force-bearing conditions. Authors - Joleen S Cheah View Article
human	IF	medRxiv Antigenic responses are hallmarks of fibrotic interstitial lung diseases independent of underlying etiologies Authors - Young Me Yoon View Article
mouse	WB,IF	Circ Res GSK-3β Localizes to the Cardiac Z-Disc to Maintain Length Dependent Activation Authors - Marisa J Stachowski-Doll View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Joleen (Verified Customer) (05-29-2019)

ABLIM should be around 87kD, but the 2 prominent bands do not come near that molecular weight. The top band is just above the 100kD molecular weight marker and the lower band is below the 75kD molecular weight marker. (BioRad Precision Plus Protein Standard used).

Applications: Western Blot,
Primary Antibody Dilution: 1:1000
Cell Tissue Type: MDCK GII

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ABLIM1 Polyclonal antibody

ABLIM1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Validation Data Gallery

WB analysis of HeLa using 15129-1-AP

WB analysis of HEK-293 using 15129-1-AP

WB analysis of NCI-H1299 using 15129-1-AP

WB analysis of HeLa using 15129-1-AP

IHC staining of human liver using 15129-1-AP

IHC staining of human liver using 15129-1-AP

IF Staining of mouse skeletal muscle using 15129-1-AP

IF Staining of mouse skeletal muscle using 15129-1-AP

IF Staining of HEK-293 using 15129-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.

Actin binding LIM 1 (abLIM1) negatively controls osteoclastogenesis by regulating cell migration and fusion.

Rictor promotes cell migration and actin polymerization through regulating ABLIM1 phosphorylation in Hepatocellular Carcinoma.

Spatial proximity of proteins surrounding zyxin under force-bearing conditions.

Antigenic responses are hallmarks of fibrotic interstitial lung diseases independent of underlying etiologies

GSK-3β Localizes to the Cardiac Z-Disc to Maintain Length Dependent Activation

Reviews