smooth muscle actin specific Polyclonal antibody

Name: smooth muscle actin specific Polyclonal antibody
Brand: Proteintech
SKU: 55135-1-AP
Rating: 5.00 (3 reviews)

smooth muscle actin specific Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

254

Reviews (3)

Cat no : 55135-1-AP

Synonyms

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, ACTA2 Smooth muscle Actin Specific, Actin, aortic smooth muscle, Alpha actin 2, Alpha SMA, Alpha SMA Specific, a-SMA, smooth muscle actin, smooth muscle actin-specific, α SMA, α-sma

Validation Data Gallery

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AllWBIHCIF-P

WB analysis using 55135-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55135-1-AP (smooth muscle actin-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 55135-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55135-1-AP (smooth muscle actin specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 55135-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human heart using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:100 (under 40x lens).
IHC staining of mouse heart using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55135-1-AP (smooth muscle actin specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55135-1-AP (smooth muscle actin specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55135-1-AP (smooth muscle actin specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human heart using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:200 (under 40x lens).
IHC staining of rat colon using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:100 (under 10x lens).
IHC staining of rat colon using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:100 (under 40x lens).
IHC staining of human hepatocirrhosis using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human hepatocirrhosis using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human colon cancer using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:2000 (under 10x lens).
IHC staining of human colon cancer using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:2000 (under 40x lens).
IHC staining of human colon using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:2000 (under 10x lens).
IHC staining of human colon using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:2000 (under 40x lens).
IHC staining of mouse colon using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:2000 (under 10x lens).
IHC staining of mouse colon using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:2000 (under 40x lens).
IHC staining of mouse liver using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon using 55135-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:50 (under 40x lens).
IF Staining of mouse heart using 55135-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse heart using 55135-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 55135-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse colon tissue, mouse kidney tissue, mouse stomach, rat colon, mouse lung, rat lung
Positive IHC detected in	mouse heart tissue, human colon tissue, human colon cancer tissue, human heart tissue, human hepatocirrhosis tissue, human liver cancer tissue, mouse colon tissue, mouse liver tissue, rat colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse heart tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:4000
Immunohistochemistry (IHC)	IHC : 1:500-1:3000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 171 publications below
IHC	See 63 publications below
IF	See 110 publications below

Product Information

55135-1-AP targets smooth muscle actin specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, rabbit
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	actin, alpha 2, smooth muscle, aorta
Calculated molecular weight	42 kDa
Observed molecular weight	42 kDa
GenBank accession number	NM_001613
Gene symbol	Alpha smooth muscle actin
Gene ID (NCBI)	59
RRID	AB_10949628
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ACTA2, also named as a-SMA,AAT6;ACTSA belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6). The immunogen of this antibody is a specific domain of ACTA2. This antibody stains the aorta only.

Protocols

Product Specific Protocols
WB protocol for smooth muscle actin specific antibody 55135-1-AP	Download protocol
IHC protocol for smooth muscle actin specific antibody 55135-1-AP	Download protocol
IF protocol for smooth muscle actin specific antibody 55135-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	ACS Nano Cysteine-Based Redox-Responsive Nanoparticles for Fibroblast-Targeted Drug Delivery in the Treatment of Myocardial Infarction Authors - Xiaoqian Ji View Article
human	IHC	Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer Authors - Xingbin Hu View Article
mouse	IF	Nat Commun Copper sulfide nanoparticles as a photothermal switch for TRPV1 signaling to attenuate atherosclerosis. Authors - Wen Gao View Article
human	IF	Mol Cancer Extracellular vesicles of carcinoma-associated fibroblasts creates a pre-metastatic niche in the lung through activating fibroblasts. Authors - Jing Kong View Article
mouse	WB,IF	J Nanobiotechnology Evolocumab loaded Bio-Liposomes for efficient atherosclerosis therapy Authors - Zhenxian Li View Article
human,mouse	WB,IF	Aging Dis HIF-1α promotes the migration and invasion of cancer-associated fibroblasts by miR-210 Authors - Ying Yang View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH S (Verified Customer) (09-23-2024)	Excelllent Applications: Western Blot Primary Antibody Dilution: 1000 Cell Tissue Type: A549 smooth muscle actin specific Antibody Western Blot validation (1000 dilution) in A549 (Cat no:55135-1-AP)
FH PK (Verified Customer) (03-22-2022)	Applications: Western Blot Cell Tissue Type: cardiac Fibroblast smooth muscle actin specific Antibody Western Blot validation ( dilution) in cardiac Fibroblast (Cat no:55135-1-AP)
FH S (Verified Customer) (12-01-2021)	Excellent Applications: Western Blot Cell Tissue Type: LUNG FIBROBLASTS smooth muscle actin specific Antibody Western Blot validation ( dilution) in LUNG FIBROBLASTS (Cat no:55135-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

smooth muscle actin specific Polyclonal antibody

smooth muscle actin specific Polyclonal Antibody for WB, IHC, IF-P, ELISA

Validation Data Gallery

WB analysis using 55135-1-AP

WB analysis using 55135-1-AP

WB analysis of mouse kidney using 55135-1-AP

IHC staining of human heart using 55135-1-AP

IHC staining of human heart using 55135-1-AP

IHC staining of mouse heart using 55135-1-AP

IHC staining of human liver cancer using 55135-1-AP

IHC staining of human liver cancer using 55135-1-AP

IHC staining of human heart using 55135-1-AP

IHC staining of human heart using 55135-1-AP

IHC staining of rat colon using 55135-1-AP

IHC staining of rat colon using 55135-1-AP

IHC staining of human hepatocirrhosis using 55135-1-AP

IHC staining of human hepatocirrhosis using 55135-1-AP

IHC staining of human colon cancer using 55135-1-AP

IHC staining of human colon cancer using 55135-1-AP

IHC staining of human colon using 55135-1-AP

IHC staining of human colon using 55135-1-AP

IHC staining of mouse colon using 55135-1-AP

IHC staining of mouse colon using 55135-1-AP

IHC staining of mouse liver using 55135-1-AP

IHC staining of mouse liver using 55135-1-AP

IHC staining of human colon using 55135-1-AP

IHC staining of human colon using 55135-1-AP

IF Staining of mouse heart using 55135-1-AP

IF Staining of mouse heart using 55135-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Cysteine-Based Redox-Responsive Nanoparticles for Fibroblast-Targeted Drug Delivery in the Treatment of Myocardial Infarction

Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer

Copper sulfide nanoparticles as a photothermal switch for TRPV1 signaling to attenuate atherosclerosis.

Extracellular vesicles of carcinoma-associated fibroblasts creates a pre-metastatic niche in the lung through activating fibroblasts.

Evolocumab loaded Bio-Liposomes for efficient atherosclerosis therapy

HIF-1α promotes the migration and invasion of cancer-associated fibroblasts by miR-210

Reviews

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