Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, MCF-7 cells, Raji cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 11 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
23226-1-AP targets ATG2A in WB, IHC, IF/ICC, IP, CoIP, ELISA, Peptide array applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ATG2A fusion protein Ag19709 相同性解析による交差性が予測される生物種 |
| Full Name | ATG2 autophagy related 2 homolog A (S. cerevisiae) |
| Calculated molecular weight | 1938 aa, 213 kDa |
| Observed molecular weight | 215 kDa |
| GenBank accession number | BC110650 |
| Gene Symbol | ATG2A |
| Gene ID (NCBI) | 23130 |
| RRID | AB_2879235 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q2TAZ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Autophagy-related protein 2 homolog A (ATG2A) belongs to the ATG2 family. ATG2A is a lipid transfer protein involved in autophagosome assembly (PMID:31271352). ATG2A tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion. ATG2A binds to the ER exit site (ERES), which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source and transfers them to ATG9 (ATG9A or ATG9B) to the IM for membrane expansion (PMID: 30952800, PMID: 31271352). ATG2A also regulates lipid droplet morphology and distribution within the cell (PMID: 28561066). The observed MW of ATG2A is 215 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATG2A antibody 23226-1-AP | Download protocol |
| IHC protocol for ATG2A antibody 23226-1-AP | Download protocol |
| IF protocol for ATG2A antibody 23226-1-AP | Download protocol |
| IP protocol for ATG2A antibody 23226-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol ATG9A protects the plasma membrane from programmed and incidental permeabilization.
| ||
Mol Cell ATG9A and ATG2A form a heteromeric complex essential for autophagosome formation | ||
Nat Commun The autophagy protein ATG9A enables lipid mobilization from lipid droplets.
| ||
EMBO Rep A conserved ATG2-GABARAP family interaction is critical for phagophore formation. | ||
Theranostics The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer. |