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ATG5 Polyclonal antibody
ATG5 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (7)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
323
Cat no : 10181-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, mouse kidney tissue, HepG2 cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 45 publications below |
| WB | See 297 publications below |
| IHC | See 27 publications below |
| IF | See 26 publications below |
| IP | See 1 publications below |
| FC | See 2 publications below |
Product Information
10181-2-AP targets ATG5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey, chicken, bovine, hamster, goat, grouper |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ATG5 fusion protein Ag0214 相同性解析による交差性が予測される生物種 |
| Full Name | ATG5 autophagy related 5 homolog (S. cerevisiae) |
| Calculated molecular weight | 32 kDa |
| Observed molecular weight | 32 kDa, 40-45 kDa, 50-55 kDa |
| GenBank accession number | BC002699 |
| Gene symbol | ATG5 |
| Gene ID (NCBI) | 9474 |
| RRID | AB_2062045 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band. 10181-2-AP also recognizes the free ATG5 (32 kDa).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATG5 antibody 10181-2-AP | Download protocol |
| IHC protocol for ATG5 antibody 10181-2-AP | Download protocol |
| IP protocol for ATG5 antibody 10181-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy | ||
Cell Res Degradation of lipid droplets by chimeric autophagy-tethering compounds.
| ||
Mol Cell Syntaxin 13, a Genetic Modifier of Mutant CHMP2B in Frontotemporal Dementia, Is Required for Autophagosome Maturation.
| ||
Adv Sci (Weinh) SETDB1 Methylates MCT1 Promoting Tumor Progression by Enhancing the Lactate Shuttle | ||
Autophagy Autophagy mediates the clearance of oligodendroglial SNCA/alpha-synuclein and TPPP/p25A in multiple system atrophy models | ||
J Clin Invest Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Priya (Verified Customer) (08-09-2023) | Used for Caco2 cells and mice colon tissue
|
FH Kamal (Verified Customer) (06-29-2023) | Works well with TBST. Atg5-12 complex (56 kDa) and Atg5 (33kDa) bands came out nice and clear.
|
FH Priya (Verified Customer) (06-21-2023) | Used this antibody for Caco2 cells andmice tissue
|
FH Priya (Verified Customer) (06-21-2023) | Used this antibody for Caco2 cells and mice tissue
|
FH Benjamin (Verified Customer) (01-07-2020) | Works well in western blot in 3% milk TBS-T
|
