Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)CoIP

WB analysis using 14418-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 14418-1-AP
37°C incubated HEK-293 membrane extract lysate were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
WB analysis using 14418-1-AP
37°C incubated mouse brain total extract lysate and membrane extract lysate were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14418-1-AP
WB result of ATP1A1 antibody (14418-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP1A1 transfected HEK-293 cells.
IP experiment of HEK-293 using 14418-1-AP
IP result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:14418-1-AP 1:20000) with HEK-293 cells lysate 1470 ug.
IP experiment of A431 using 14418-1-AP
IP result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:14418-1-AP 1:5000) with A431 cells lysate 3200ug.
IHC staining of human liver cancer using 14418-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human ovary tumor using 14418-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP (ATP1A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 14418-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 14418-1-AP (ATP1A1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 14418-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 14418-1-AP (ATP1A1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14418-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human ovary tumor using 14418-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP (ATP1A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 14418-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14418-1-AP (ATP1A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 14418-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human ovary tumor using 14418-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of Caco-2 using 14418-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed Caco-2 cells using ATP1A1 antibody (14418-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HEK-293 using 14418-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using ATP1A1 antibody (14418-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HEK-293 using 14418-1-AP
1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug ATP1A1 Polyclonal antibody (14418-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
CoIP experiment of A431 using 14418-1-AP
Co-IP result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:15192-1-AP 1:5000) with A431 cells lysate 1000ug.

Featured Product
KD/KO Validated

ATP1A1 Polyclonal antibody

ATP1A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Cat No. 14418-1-AP

Publications(218)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

Na+/K+ ATPase 1, Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13, Atpa 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (218)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse heart tissue, HEK-293 cells, 37°C incubated HEK-293 cells, 37°C incubated mouse brain tissue, rat heart tissue
Positive IP detected in	HEK-293 cells, A431 cells
Positive IHC detected in	human ovary tumor tissue, human cervical cancer tissue, human colon tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Caco-2 cells, HEK-293 cells
Positive FC (Intra) detected in	HEK-293 cells
Positive CoIP detected in	A431 cells

For optimal WB detection with 14418-1-AP, we do not recommend boiling the sample after lysis.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:20000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Co-Immunoprecipitation (CoImmunoprecipitation (IP))	COIP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 198 publications below
IHC	See 8 publications below
IF	See 23 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

14418-1-AP targets ATP1A1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ATP1A1 fusion protein Ag5763 相同性解析による交差性が予測される生物種
Full Name	ATPase, Na+/K+ transporting, alpha 1 polypeptide
Calculated molecular weight	113 kDa
Observed molecular weight	100-110 kDa
GenBank accession number	BC050359
Gene Symbol	ATP1A1
Gene ID (NCBI)	476
ENSEMBL Gene ID	ENSG00000163399
RRID	AB_2227873
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P05023
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

Protocols

Product Specific Protocols
WB protocol for ATP1A1 antibody 14418-1-AP	Download protocol
IHC protocol for ATP1A1 antibody 14418-1-AP	Download protocol
IF protocol for ATP1A1 antibody 14418-1-AP	Download protocol
IP protocol for ATP1A1 antibody 14418-1-AP	Download protocol
FC protocol for ATP1A1 antibody 14418-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Nature Low-dose metformin targets the lysosomal AMPK pathway through PEN2. Authors - Teng Ma View Article
mouse	WB	Immunity Disruption of the Na+/K+-ATPase-purinergic P2X7 receptor complex in microglia promotes stress-induced anxiety Authors - Songqiang Huang View Article
	WB	Adv Mater Ultrasound Imaging of Tumor Vascular CD93 with MMRN2 Modified Microbubbles for Immune Microenvironment Prediction Authors - Dingyi Wang View Article
mouse	WB	ACS Nano Biomimetic Nanomedicine Targeting Orchestrated Metabolism Coupled with Regulatory Factors to Disrupt the Metabolic Plasticity of Breast Cancer Authors - Lingtong Meng View Article
	WB	Adv Sci (Weinh) Ultrasound Responsive Nanovaccine Armed with Engineered Cancer Cell Membrane and RNA to Prevent Foreseeable Metastasis Authors - Wenqi Sun View Article
human	WB	Theranostics Stiff matrix induced srGAP2 tension gradients control migration direction in triple-negative breast cancer Authors - Chen Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14418-1-AP

WB analysis using 14418-1-AP

WB analysis using 14418-1-AP

WB analysis of HEK-293 using 14418-1-AP

IP experiment of HEK-293 using 14418-1-AP

IP experiment of A431 using 14418-1-AP

IHC staining of human liver cancer using 14418-1-AP

IHC staining of human ovary tumor using 14418-1-AP

IHC staining of human colon using 14418-1-AP

IHC staining of human colon using 14418-1-AP

IHC staining of human liver cancer using 14418-1-AP

IHC staining of human ovary tumor using 14418-1-AP

IHC staining of human cervical cancer using 14418-1-AP

IHC staining of human ovary tumor using 14418-1-AP

IHC staining of human ovary tumor using 14418-1-AP

IF Staining of Caco-2 using 14418-1-AP

IF Staining of HEK-293 using 14418-1-AP

FC experiment of HEK-293 using 14418-1-AP

CoIP experiment of A431 using 14418-1-AP

ATP1A1 Polyclonal antibody

ATP1A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

-/ -

Tested Applications

For optimal WB detection with 14418-1-AP, we do not recommend boiling the sample after lysis.

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Low-dose metformin targets the lysosomal AMPK pathway through PEN2.

Disruption of the Na+/K+-ATPase-purinergic P2X7 receptor complex in microglia promotes stress-induced anxiety

Ultrasound Imaging of Tumor Vascular CD93 with MMRN2 Modified Microbubbles for Immune Microenvironment Prediction

Biomimetic Nanomedicine Targeting Orchestrated Metabolism Coupled with Regulatory Factors to Disrupt the Metabolic Plasticity of Breast Cancer

Ultrasound Responsive Nanovaccine Armed with Engineered Cancer Cell Membrane and RNA to Prevent Foreseeable Metastasis

Stiff matrix induced srGAP2 tension gradients control migration direction in triple-negative breast cancer