AuthentiKine™ Human IL-17A ELISA Kit
Cat no : KE00203
Synonyms
CTLA 8, CTLA8, IL 17, IL 17A, IL17, IL17A, IL-17A, interleukin 17A
Validation Data Gallery
Product Information
KE00203 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-17A ELISA kit is to be used to detect and quantify protein levels of endogenous IL-17A. The assay recognizes human IL-17A. An antibody specific for IL-17A has been pre-coated onto the microwells. The IL-17A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL-17A is added to detect the captured IL-17A protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human IL-17A ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.4 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3605 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 102% | 90%-116% |
| Human Plasma | 90% | 74%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 543.2 | 13.4 | 2.5 |
| 2 | 20 | 129.4 | 5.5 | 4.2 |
| 3 | 20 | 30.1 | 1.9 | 6.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 555.2 | 31.4 | 5.7 |
| 2 | 24 | 141.8 | 8.4 | 5.9 |
| 3 | 24 | 28 | 2.5 | 9.0 |
Background Information
IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CTLA 8, CTLA8, IL 17, IL 17A, IL17, IL17A, IL-17A, interleukin 17A |
Publications
| Species | Sample Type | Title |
|---|---|---|
J Invest Dermatol UBE2L3 Reduces TRIM21 Expression and Interleukin-1β Secretion in Epidermal Keratinocytes and Improves Psoriasis-like Skin | ||
J Neuroimmunol Calcitonin gene-related peptide upregulates IL-17A and IL-22 in γδ-T cells through the paracrine effect of langerhans cells on LC/γδ-T co-culture model | ||
Oxid Med Cell Longev lncRNA CRNDE Affects Th17/IL-17A and Inhibits Epithelial-Mesenchymal Transition in Lung Epithelial Cells Reducing Asthma Signs | ||
J Dermatol Sci Topical histone deacetylase 1 inhibitor Entinostat ameliorates psoriasiform dermatitis through suppression of IL-17A response | ||
J Dermatol Sci Rh family C glycoprotein contributes to psoriatic inflammation through regulating the dysdifferentiation and cytokine secretion of keratinocytes |