BAP1 Polyclonal antibody

Name: BAP1 Polyclonal antibody
Brand: Proteintech
SKU: 10398-1-AP

BAP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10398-1-AP

Synonyms

BAP1, BRCA1 associated protein 1, Cerebral protein 6, HUCEP 13, hucep 6, KIAA0272, UCHL2

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of A2780 using 10398-1-AP
A2780 cells were subjected to SDS PAGE followed by western blot with 10398-1-AP (BAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 10398-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10398-1-AP (BAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 10398-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 10398-1-AP (BAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10398-1-AP
IP result of anti-BAP1 (IP:10398-1-AP, 4ug; Detection:10398-1-AP 1:300) with HeLa cells lysate 3000ug.
IHC staining of human ovary tumor using 10398-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10398-1-AP (BAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 10398-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10398-1-AP (BAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10398-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10398-1-AP (BAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10398-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10398-1-AP (BAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 10398-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A2780 cells, human placenta tissue, mouse ovary tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human ovary tumor tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below

Product Information

10398-1-AP targets BAP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	BAP1 fusion protein Ag0597 相同性解析による交差性が予測される生物種
Full Name	BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase)
Calculated molecular weight	81-91 kDa
Observed molecular weight	91 kDa
GenBank accession number	BC001596
Gene symbol	BAP1
Gene ID (NCBI)	8314
RRID	AB_2180460
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BRCA1-associated protein-1 (BAP1),also named as KIAA0272, hucep-6, DKFZp686N04275, FLJ35406, FLJ37180, HUCEP-13, is a deubiquitinating enzyme whose function in the control of the cell cycle requires both its deubiquitinating activity and nuclear localization(PMID:22374320). It helps to control cell proliferation by regulating HCFC1 protein levels and by associating with genes involved in the G(1)-S transition)(PMID:19188440). Defects in BAP1 may be a cause of mesothelioma malignant (MESOM)(PMID:21642991) and tumor predisposition syndrome (TPDS)(PMID:21874003). The all BAP1s except those of C. quinquefasciatus have a molecular mass of more than 90 kDa(PMID:22374320).

Protocols

Product Specific Protocols
WB protocol for BAP1 antibody 10398-1-AP	Download protocol
IHC protocol for BAP1 antibody 10398-1-AP	Download protocol
IP protocol for BAP1 antibody 10398-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Immunol Res USP22 deubiquitinates CD274 to suppress anti-cancer immunity. Authors - Xing Huang View Article
human	WB	iScience Centrosomal protein 120 promotes centrosome amplification and gastric cancer progression via USP54-mediated deubiquitination of PLK4 Authors - Chenggang Zhang View Article
mouse	WB	Nature Basis of the H2AK119 specificity of the Polycomb repressive deubiquitinase Authors - Weiran Ge View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BAP1 Polyclonal antibody

BAP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of A2780 using 10398-1-AP

WB analysis of mouse ovary using 10398-1-AP

WB analysis of human placenta using 10398-1-AP

IP experiment of HeLa using 10398-1-AP

IHC staining of human ovary tumor using 10398-1-AP

IHC staining of human ovary tumor using 10398-1-AP

IHC staining of human lung cancer using 10398-1-AP