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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BOK Polyclonal antibody

BOK Polyclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 26430-1-AP

Synonyms

BCL2L9, hBOK, BOKL, Bcl-2-related ovarian killer protein, Bcl-2-like protein 9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BOK Polyclonal antibody (26430-1-AP)

    WB analysis using 26430-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26430-1-AP (BOK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of MCF-7 cells using BOK Polyclonal antibody (26430-1-AP)

    FC experiment of MCF-7 using 26430-1-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human BOK (26430-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHepG2 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26430-1-AP targets BOK in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BOK fusion protein Ag24070 相同性解析による交差性が予測される生物種
Full Name BCL2-related ovarian killer
Calculated molecular weight 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC006203
Gene symbol BOK
Gene ID (NCBI) 666
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BOK belongs to the BCL2 family, members of which form homo- or heterodimers, and act as anti- or proapoptotic regulators that are involved in a wide variety of cellular processes. Studies in rat show that this protein has restricted expression in reproductive tissues, interacts strongly with some antiapoptotic BCL2 proteins, not at all with proapoptotic BCL2 proteins, and induces apoptosis in transfected cells. Thus, this protein represents a proapoptotic member of the BCL2 family.

Protocols

Product Specific Protocols
WB protocol for BOK antibody 26430-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols