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KD/KO Validated

RtcB-Specific Polyclonal antibody

Name: RtcB-Specific Polyclonal antibody
Brand: Proteintech
SKU: 19809-1-AP

RtcB-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 19809-1-AP

Synonyms

C22orf28, FAAP, focal adhesion associated protein, hematopoietic stem/progenitor cell 117, HSPC117, RtcB, RtcB-Specific

Validation Data Gallery

Applications:

AllWBIPIHCIF

WB analysis using 19809-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19809-1-AP (RtcB-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 19809-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19809-1-AP (RtcB-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 19809-1-AP
IP result of anti-RtcB-Specific (IP:19809-1-AP, 3ug; Detection:19809-1-AP 1:500) with HeLa cells lysate 3000ug.
IP experiment of HeLa using 19809-1-AP
IP result of anti-RtcB-Specific (IP:19809-1-AP, 4ug; Detection:19809-1-AP 1:4000) with HeLa cells lysate 1800 ug.
IHC staining of human lung cancer using 19809-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19809-1-AP (RtcB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with RTCB antibody included
IHC staining of human stomach cancer using 19809-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19809-1-AP (RtcB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with RTCB antibody included
IHC staining of human liver cancer using 19809-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19809-1-AP (RtcB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with RTCB antibody included
IF Staining of HeLa using 19809-1-AP
Immunofluorescent analysis of HeLa cells using 19809-1-AP (RtcB-Specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 19809-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, mouse brain tissue, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human lung cancer tissue, human liver cancer tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy RTCB Ready-To-Use IHC Kit. RTCB primary antibody included.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)	IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IP	See 2 publications below

Product Information

19809-1-AP targets RtcB-Specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RtcB-Specific fusion protein Ag13836 相同性解析による交差性が予測される生物種
Full Name	chromosome 22 open reading frame 28
Calculated molecular weight	55 kDa
Observed molecular weight	55 kDa
GenBank accession number	BC000151
Gene symbol	C22orf28
Gene ID (NCBI)	51493
RRID	AB_10695047
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RTCB (also known as HSPC117, C22orf28, FAAP and D10Wsu52e) is an essential subunit of a tRNA ligase complex that is involved in RNA repair and stress-induced splicing. As a multifunctional protein, RTCB is also a cell adhesion protein and is important in embryo and placenta development. Recently RTCB has been identified as UPR RNA ligase catalyzing unconventional XBP1 mRNA splicing. This antibody specifically recognizes endogenous RTCB protein. (25087875)

Protocols

Product Specific Protocols
WB protocol for RtcB-Specific antibody 19809-1-AP	Download protocol
IHC protocol for RtcB-Specific antibody 19809-1-AP	Download protocol
IF protocol for RtcB-Specific antibody 19809-1-AP	Download protocol
IP protocol for RtcB-Specific antibody 19809-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell ERα is an RNA-binding protein sustaining tumor cell survival and drug resistance. Authors - Yichen Xu View Article
human	WB,IP	Mol Cell A Synthetic Biology Approach Identifies the Mammalian UPR RNA Ligase RtcB. Authors - Yanyan Lu View Article
pig	WB	Nat Commun Reprogramming of microRNA expression via E2F1 downregulation promotes Salmonella infection both in infected and bystander cells. Authors - Carmen Aguilar View Article
human	WB	J Biol Chem The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress. Authors - Irem Unlu KO Validated View Article
human	IP	Life Sci Alliance Stress-induced tyrosine phosphorylation of RtcB modulates IRE1 activity and signaling outputs. Authors - Alexandra Papaioannou View Article
human	WB	J Immunol The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1 Authors - Lei Cao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RtcB-Specific Polyclonal antibody

RtcB-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis using 19809-1-AP

WB analysis of mouse brain using 19809-1-AP

IP experiment of HeLa using 19809-1-AP

IP experiment of HeLa using 19809-1-AP

IHC staining of human lung cancer using 19809-1-AP

IHC staining of human stomach cancer using 19809-1-AP

IHC staining of human liver cancer using 19809-1-AP

IF Staining of HeLa using 19809-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ERα is an RNA-binding protein sustaining tumor cell survival and drug resistance.

A Synthetic Biology Approach Identifies the Mammalian UPR RNA Ligase RtcB.

Reprogramming of microRNA expression via E2F1 downregulation promotes Salmonella infection both in infected and bystander cells.

The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress.

Stress-induced tyrosine phosphorylation of RtcB modulates IRE1 activity and signaling outputs.

The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1