Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 13198-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13198-2-AP (CA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 13198-2-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 13198-2-AP (CA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 13198-2-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13198-2-AP (CA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of rat spleen using 13198-2-AP
rat spleen tissue were subjected to SDS PAGE followed by western blot with 13198-2-AP (CA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 13198-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13198-2-AP (CA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 13198-2-AP
IP result of anti-CA1 (IP:13198-2-AP, 4ug; Detection:13198-2-AP 1:500) with K-562 cells lysate 1200ug.
IHC staining of human breast cancer using 13198-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13198-2-AP (CA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 13198-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13198-2-AP (CA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 13198-2-AP
Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 13198-2-AP (CA1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

Carbonic anhydrase 1/CA1 Polyclonal antibody

Carbonic anhydrase 1/CA1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13198-2-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

CA1, CA I, Carbonate dehydratase I, Car1, CA-I

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse spleen tissue, Jurkat cells, rat spleen tissue
Positive IP detected in	K-562 cells
Positive IHC detected in	human breast cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IHC	See 1 publications below
IF	See 4 publications below
IP	See 1 publications below

Product Information

13198-2-AP targets Carbonic anhydrase 1/CA1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Carbonic anhydrase 1/CA1 fusion protein Ag3932 相同性解析による交差性が予測される生物種
Full Name	carbonic anhydrase I
Calculated molecular weight	261 aa, 29 kDa
Observed molecular weight	29 kDa
GenBank accession number	BC027890
Gene Symbol	CA1
Gene ID (NCBI)	759
RRID	AB_2275041
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P00915
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CA1(Carbonic anhydrase 1) is also named as CAB and belongs to the alpha-carbonic anhydrase family, which may reside in cytoplasm, in mitochondria, or in secretory granules, or associate with membranes in cell. It forms a large family of genes encoding zinc metalloenzymes of great physiologic importance. Extracellular CA1 mediates hemorrhagic retinal and cerebral vascular permeability through prekallikrein activation(PMID:17259996).

Protocols

Product Specific Protocols
WB protocol for Carbonic anhydrase 1/CA1 antibody 13198-2-AP	Download protocol
IHC protocol for Carbonic anhydrase 1/CA1 antibody 13198-2-AP	Download protocol
IP protocol for Carbonic anhydrase 1/CA1 antibody 13198-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,rat	WB,IF	Acta Neuropathol Commun Upregulation of carbonic anhydrase 1 beneficial for depressive disorder Authors - Ke Cheng KO Validated View Article
rat	WB	J Ginseng Res Ginsenoside Rg1 attenuates mechanical stress-induced cardiac injury via calcium sensing receptor-related pathway Authors - Mei-Li Lu View Article
mouse	IF	FASEB J Loss of interleukin-10 receptor disrupts intestinal epithelial cell proliferation and skews differentiation towards the goblet cell fate. Authors - Brittany R Jenkins View Article
human	WB	J Inflamm Res M1-Type Macrophages Secrete TNF-α to Stimulate Vascular Calcification by Upregulating CA1 and CA2 Expression in VSMCs Authors - Xianqin Song View Article
mouse	WB	Am J Physiol Lung Cell Mol Physiol Carbonic anhydrase and soluble adenylate cyclase regulation of cystic fibrosis cellular phenotypes. Authors - Kathleen Boyne View Article
human,rat	WB	Front Pharmacol Calcium Sensing Receptor-Related Pathway Contributes to Cardiac Injury and the Mechanism of Astragaloside IV on Cardioprotection. Authors - Meili Lu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13198-2-AP

WB analysis of Jurkat using 13198-2-AP

WB analysis of mouse spleen using 13198-2-AP

WB analysis of rat spleen using 13198-2-AP

WB analysis using 13198-2-AP

IP experiment of K-562 using 13198-2-AP

IHC staining of human breast cancer using 13198-2-AP

IHC staining of human breast cancer using 13198-2-AP

IHC staining of human colon cancer using 13198-2-AP

Carbonic anhydrase 1/CA1 Polyclonal antibody

Carbonic anhydrase 1/CA1 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Upregulation of carbonic anhydrase 1 beneficial for depressive disorder

Ginsenoside Rg1 attenuates mechanical stress-induced cardiac injury via calcium sensing receptor-related pathway

Loss of interleukin-10 receptor disrupts intestinal epithelial cell proliferation and skews differentiation towards the goblet cell fate.

M1-Type Macrophages Secrete TNF-α to Stimulate Vascular Calcification by Upregulating CA1 and CA2 Expression in VSMCs

Carbonic anhydrase and soluble adenylate cyclase regulation of cystic fibrosis cellular phenotypes.

Calcium Sensing Receptor-Related Pathway Contributes to Cardiac Injury and the Mechanism of Astragaloside IV on Cardioprotection.