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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CA11 Polyclonal antibody

CA11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15435-1-AP

Synonyms

CA RP II, CA RP XI, CA XI, CA11, carbonic anhydrase XI, CARP 2, CARP XI, CARP2, CARPX1

Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using CA11 Polyclonal antibody (15435-1-AP)

    WB analysis of human kidney using 15435-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15435-1-AP (CA11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using CA11 Polyclonal antibody (15435-1-AP)

    WB analysis of human kidney using 15435-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15435-1-AP (CA11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using CA11 Polyclonal antibody (15435-1-AP)

    IHC staining of human brain using 15435-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using CA11 Polyclonal antibody (15435-1-AP)

    IHC staining of human brain using 15435-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CA11 Polyclonal antibody (15435-1-AP)

    IHC staining of human placenta using 15435-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CA11 Polyclonal antibody (15435-1-AP)

    IHC staining of human placenta using 15435-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CA11 Polyclonal antibody (15435-1-AP)

    IHC staining of human testis using 15435-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CA11 Polyclonal antibody (15435-1-AP)

    IHC staining of human testis using 15435-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CA11 Polyclonal antibody (15435-1-AP)

    IHC staining of human spleen using 15435-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CA11 Polyclonal antibody (15435-1-AP)

    IHC staining of human spleen using 15435-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using CA11 Polyclonal antibody (15435-1-AP)

    IHC staining of human lung using 15435-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using CA11 Polyclonal antibody (15435-1-AP)

    IHC staining of human lung using 15435-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using CA11 Polyclonal antibody (15435-1-AP)

    IHC staining of human ovary using 15435-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using CA11 Polyclonal antibody (15435-1-AP)

    IHC staining of human ovary using 15435-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inhuman kidney tissue
Positive IHC detected inhuman brain tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15435-1-AP targets CA11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA11 fusion protein Ag7712 相同性解析による交差性が予測される生物種
Full Name carbonic anhydrase XI
Calculated molecular weight 36 kDa
Observed molecular weight 36 kda, 45 kDa
GenBank accession numberBC002662
Gene symbol CA11
Gene ID (NCBI) 770
RRIDAB_2065700
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CA11(Carbonic anhydrase-related protein 11) is also named as CARP2, CARP XI and belongs to the alpha-carbonic anhydrase family. CARP XI is observed in the cerebellum, cerebrum, liver, stomach, small intestine, colon, kidney, and testis by immunohistochemical and in the cerebellum, the most prominent signal is located in the Purkinje cells(PMID:20356370). CARP XI contains a hydrophobic N-terminal region for a possible signal sequence and asparagine glycosylation site and it plays a general role in the human central nervous system(PMID: 10350627).

Protocols

Product Specific Protocols
WB protocol for CA11 antibody 15435-1-APDownload protocol
IHC protocol for CA11 antibody 15435-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols